Electrochemical oxidation of NADH on sol–gel derived, surface renewable, non-modified and mediator modified composite-carbon electrodes

The electrochemical oxidation of the cofactor, dihydronicotinamide adenine dinucleotide was studied on sol–gel derived, polishable, ceramic-carbon electrodes (CCEs), in phosphate buffer of pH 7.3. NADH was found to undergo an apparently irreversible, two-electron oxidation in the available range of potentials. Cyclic voltammetric studies at high sweep rates revealed a prewave due to the adsorption of the oxidation product, NAD+. The prewave was...