Activation of Ca2+/Calmodulin-dependent Protein Kinase (CaM-kinase) IV by CaM-kinase Kinase in Jurkat T Lymphocytes

Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV), a member of the CaM-kinase family involved in transcriptional regulation, is stimulated by Ca2+/CaM but also requires phosphorylation by a CaM-kinase kinase for full activation. In this study we investigated the physiological role of a CaM-kinase cascade in Jurkat T human lymphocytes through antigen receptor (CD3) signaling. Total and Ca2+-independent CaM-kinase IV activities were...