Thiamin requirement of juvenile abalone, Haliotis discus hannai Ino

Juvenile abalone, Haliotis discus hannai Ino, of initial weight 0.55±0.02 g, were fed with experimental diets with graded levels of thiamin–HCl (0, 9.8, 18.9, 37.2, 75.9, 150.4 and 309.5 mg/kg diet) for 16 weeks. Thiamin–HCl and other water-soluble vitamins were microencapsulated with sodium alginate by an emulsion coacervation process prior to supplementation. To estimate the thiamin requirement of abalone and to evaluate the thiamin status in...