Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins

A fusion protein comprising a TALE DNA-targeting domain and the 5-methylcytosine hydroxylase TET1 enables investigation of the function of specific DNA methylation events. Genome-wide studies have defined cell type–specific patterns of DNA methylation1 that are important for regulating gene expression in both normal development2 and disease3. However, determining the functional significance of specific methylation events remains challenging,...