Efficient prefractionation of low‐abundance proteins in human plasma and construction of a two‐dimensional map

Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high‐abundance proteins that seriously interfere with the detection of low‐abundance potential biomarker proteins. To establish a high‐throughput method for efficient depletion of high‐abundance proteins and subsequent fractionation, prior to molecular analysis of proteins, we explored how...