Original paper
Genome engineering using the CRISPR-Cas9 system
Abstract
Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 20-nt targeting sequence within its guide RNA. Here we describe a set of tools for Cas9-mediated genome editing via...
Paper Details
Title
Genome engineering using the CRISPR-Cas9 system
Published Date
Oct 24, 2013
Journal
Volume
8
Issue
11
Pages
2281 - 2308
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Notes
History