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Alterations of phospholipid concentration and species composition of the intestinal mucus barrier in ulcerative colitis: A clue to pathogenesis

Published on Nov 1, 2009in Inflammatory Bowel Diseases4.00
· DOI :10.1002/ibd.20993
Annika Braun10
Estimated H-index: 10
(University Hospital Heidelberg),
Irina Treede3
Estimated H-index: 3
(University Hospital Heidelberg)
+ 11 AuthorsRobert Ehehalt13
Estimated H-index: 13
(University Hospital Heidelberg)
Abstract
Background: Phospholipids are essential for the normal function of the intestinal mucus barrier. The objective of this study was to systematically investigate phospholipids in the intestinal mucus of humans suffering from inflammatory bowel diseases, where a barrier defect is strongly supposed to be pathogenetic. Methods: Optimal mucus recovery was first validated in healthy mice and the method was then transferred to the endoscopic acquisition of ileal and colonic mucus from 21 patients with ulcerative colitis (UC), 10 patients with Crohn's disease (CD), and 29 healthy controls. Nano-electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used to determine phosphatidylcholine (PC), lysophosphatidylcholine (LPC), and sphingomyelin (SM) in lipid extracts of mucus specimens. Results: Human and rodent mucus contained very similar phospholipid species. In the ileal and colonic mucus from patients suffering from UC, the concentration of PC was highly significantly lower (607 ± 147 pmol/100 μg protein and 745 ± 148 pmol/100 μg protein) compared to that of patients with CD (3223 ± 1519 pmol/100 μg protein and 2450 ± 431 pmol/100 μg protein) and to controls (3870 ± 760 pmol/100 μg protein and 2790 ± 354 pmol/100 μg protein); overall, P = 0.0002 for ileal specimens and P < 0.0001 for colonic specimens. Independent of disease activity, patients suffering from UC showed an increased saturation grade of PC fatty acid residues and a higher LPC-to-PC ratio. Conclusions: The intestinal mucus barrier of patients with UC is significantly altered concerning its phospholipid concentration and species composition. These alterations may be very important for the pathogenesis of this disease and underline new therapeutic strategies. Inflamm Bowel Dis 2009
  • References (60)
  • Citations (90)
References60
Newest
#1Wolfgang Stremmel (University Hospital Heidelberg)H-Index: 73
#2Robert Ehehalt (University Hospital Heidelberg)H-Index: 13
Last.Max Karner (University Hospital Heidelberg)H-Index: 10
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#1Jan Wehkamp (University of Tübingen)H-Index: 42
#2Guoxing Wang (University of Tübingen)H-Index: 7
Last.Matthias Schwab (University of Tübingen)H-Index: 24
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Cited By90
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#1Urszula Daniluk (UMB: Medical University of Białystok)H-Index: 2
#2Jaroslaw Daniluk (UMB: Medical University of Białystok)H-Index: 9
Last.Michal Ciborowski (UMB: Medical University of Białystok)H-Index: 13
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#1Lei Shi (Beijing University of Chinese Medicine)H-Index: 1
#2Yi Dai (Hyogo University of Health Sciences)H-Index: 1
Last.Junxiang Li (Beijing University of Chinese Medicine)H-Index: 3
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#1Eric A. Franzosa (Broad Institute)H-Index: 23
#2Alexandra Sirota-Madi (Broad Institute)H-Index: 4
Last.Himel Mallick (Broad Institute)H-Index: 8
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#1Brigida Rusconi (WashU: Washington University in St. Louis)H-Index: 2
#2Xuntian Jiang (WashU: Washington University in St. Louis)H-Index: 20
Last.Phillip I. Tarr (WashU: Washington University in St. Louis)H-Index: 63
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