Original paper

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

Volume: 32, Issue: 12, Pages: 1262 - 1267
Published: Sep 3, 2014
Abstract
Analysis of the genome editing activity of more than 1800 sgRNAs in mouse and human cells yields rules to facilitate design of highly active RNA guides for Cas-9 genome editing. Components of the prokaryotic clustered, regularly interspaced, short palindromic repeats (CRISPR) loci have recently been repurposed for use in mammalian cells1,2,3,4,5,6. The CRISPR-associated (Cas)9 can be programmed with a single guide RNA (sgRNA) to generate...
Paper Details
Title
Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
Published Date
Sep 3, 2014
Volume
32
Issue
12
Pages
1262 - 1267
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