Match!

Propofol Reduces Apoptosis and Up-Regulates Endothelial Nitric Oxide Synthase Protein Expression in Hydrogen Peroxide-Stimulated Human Umbilical Vein Endothelial Cells

Published on Oct 1, 2007in Anesthesia & Analgesia3.49
· DOI :10.1213/01.ane.0000281046.77228.91
Baohua Wang6
Estimated H-index: 6
,
Tao Luo2
Estimated H-index: 2
+ 1 AuthorsDavid M. Ansley19
Estimated H-index: 19
Cite
Abstract
BACKGROUND:Vascular endothelial cells play an important role in maintaining cardiovascular homeostasis. Oxidative stress is a critical pathogenic factor in endothelial cell damage and the development of cardiovascular diseases. In this study we evaluated the effects of propofol on oxidative stress-i
  • References (32)
  • Citations (49)
Cite
References32
Newest
Published on Sep 1, 2006in Anesthesia & Analgesia3.49
Zhengyuan Xia32
Estimated H-index: 32
,
Zhiyong Huang3
Estimated H-index: 3
,
David M. Ansley19
Estimated H-index: 19
We investigated if increasing propofol's dosage to augment its antioxidant capacity during cardiopulmonary bypass (CPB) could confer cardiac protection. Fifty-four coronary artery bypass graft surgery patients were randomly assigned to small-dose propofol (Group P; n = 18), large-dose propofol (Group HiP; n = 18), or isoflurane Group (Group I; n = 18). After the induction, anesthesia was maintained with an inspired concentration of isoflurane 1%-3.5% (Group I) or a continuous infusion of propofo...
Published on Jul 1, 2006in Anesthesia & Analgesia3.49
Tao Luo3
Estimated H-index: 3
,
Zhengyuan Xia32
Estimated H-index: 32
We designed the present study to test the hypothesis that oxygen free radicals can enhance tumor necrosis factor (TNF)-α cellular toxicity, which might be reversed by propofol, an anesthetic with antioxidant properties, in human vascular endothelial cell line ECV304. Cultured ECV304 were either not
Published on Jul 1, 2006in European Journal of Pharmacology3.17
Ken-ichi Chikutei2
Estimated H-index: 2
(Tokushima Bunri University),
Tomohiro M. Oyama11
Estimated H-index: 11
(Tokushima Bunri University)
+ 7 AuthorsYasuooyama30
Estimated H-index: 30
(University of Tokushima)
Abstract Propofol (2,6-diisopropylphenol) is a general anesthetic possessing a neuroprotective action against oxidative stress produced by H 2 O 2 . H 2 O 2 induces an exposure of phosphatidylserine on outer surface of cell membranes, resulting in change in membrane phospholipid arrangement, in rat thymocytes. Since propofol is highly lipophilic, the agent is presumed to interact with membrane lipids and hence to modify the cell vulnerability to H 2 O 2 . Therefore, to test the possibility, we h...
Published on May 1, 2006in Anesthesiology6.42
Peter J. Wickley7
Estimated H-index: 7
,
Xueqin Ding7
Estimated H-index: 7
+ 1 AuthorsDerek S. Damron24
Estimated H-index: 24
Background: Myocardial protection by anesthetics is known to involve activation of protein kinase C (PKC). The authors’ objective was to identify the PKC isoforms activated by propofol in rat ventricular myocytes. They also assessed the intracellular location of individual PKC isoforms before and after treatment with propofol. Methods: Freshly isolated ventricular myocytes were obtained from adult rat hearts. Immunoblot analysis of cardiomyocyte subcellular fractions was used to assess transloca...
Published on May 1, 2006in Anesthesiology6.42
Peter J. Wickley7
Estimated H-index: 7
,
Toshiya Shiga12
Estimated H-index: 12
+ 1 AuthorsDerek S. Damron24
Estimated H-index: 24
Background: The authors' objective was to assess the role of protein kinase C (PKC) and nitric oxide synthase (NOS) in mediating the effects of propofol on diabetic cardiomyocyte contractility, intracellular free Ca 2+ concentration ([Ca 2+ ] i ), and myofilament Ca 2+ sensitivity. Methods: Freshly isolated ventricular myocytes were obtained from normal and diabetic rat hearts. [Ca 2+ ] i and cell shortening were simultaneously measured in electrically stimulated, ventricular myocytes using fura...
Published on Apr 1, 2006in Cell Death & Differentiation8.09
M Tesauro1
Estimated H-index: 1
(University of Rome Tor Vergata),
W. C. Thompson2
Estimated H-index: 2
(NIH: National Institutes of Health),
J. Moss2
Estimated H-index: 2
(NIH: National Institutes of Health)
Nitric oxide (NO) may block apoptosis by inhibiting caspases via S-nitrosylation of cysteines. Here, we investigated whether effector caspases might cleave and thereby inhibit endothelial nitric oxide synthase (eNOS). Exposure of eNOS-transfected COS-7 cells and bovine aortic endothelial cells to staurosporine resulted in significant loss of 135-kDa eNOS protein and activity, and appearance of a 60-kDa eNOS fragment; effects were inhibited by the general caspase inhibitor, benzyloxycarbonyl-Val-...
Published on Mar 1, 2006in Cellular Signalling3.39
Feng Ming Ho10
Estimated H-index: 10
,
Wan-Wan Lin44
Estimated H-index: 44
(NTU: National Taiwan University)
+ 6 AuthorsChiau S. Liau1
Estimated H-index: 1
(NTU: National Taiwan University)
Our previous studies demonstrated that high glucose-induced apoptosis in human umbilical vein endothelial cells (HUVECs) is mediated by sequential activation of c-Jun N-terminal kinase (JNK) and caspase, and prevented by exogenous nitric oxide (NO). In this study we further elucidated the roles of the transcriptional factor NF-κB, phosphatidylinositol 3′-kinase (PI3K), Akt and endothelial nitric oxide synthase (eNOS) in the apoptosis of HUVECs induced by high glucose. The results showed that hig...
Published on Dec 15, 2005in Cardiovascular Research7.01
Yaser Abdallah21
Estimated H-index: 21
(University of Giessen),
Claudia Schäfer18
Estimated H-index: 18
(University of Giessen)
See article by Ma et al. [4] (pages 57–65) in this issue. Protection of myocardium against lethal reperfusion injury is one of the major challenges of clinical cardiology today. Many interventions during reperfusion that may influence infarct size or improve cardiac function have been studied experimentally, but none of these has yet found its way into clinical practice. One of the oldest and most discussed interventions to modify reperfusion conditions is the use of glucose–insulin–potassium (G...
Published on Oct 1, 2005in Cardiovascular Research7.01
Hua Cai43
Estimated H-index: 43
(U of C: University of Chicago)
Increased production of reactive oxygen species (ROS) has been implicated in the pathogenesis of cardiovascular diseases. Enzymatic systems such as the mitochondrial respiratory chain, vascular NAD(P)H oxidases, xanthine oxidase, and uncoupled endothelial nitric oxide synthase (eNOS) produce superoxide anion (O2.−) in vascular cells. While some O2.− rapidly degrades by reacting with nitric oxide (NO.), the O2.− signal preserved by dismutation into hydrogen peroxide (H2O2) exerts prolonged signal...
Published on Jul 1, 2005in Journal of Neurosurgical Anesthesiology2.96
Hyung Lee28
Estimated H-index: 28
,
Young-Ho Jang6
Estimated H-index: 6
,
Seong-Ryong Lee23
Estimated H-index: 23
Abstract:This study compared the effectiveness of propofol with that of trolox and melatonin for reduction of lipid peroxidation in vitro. Lipid peroxidation was induced by addition of kainic acid (KA; 10 mM), hydrogen peroxide (H2O2; 10 mM), or ferrous ammonium sulfate (5 μM) to mouse brain homogen
Cited By49
Newest
Published on May 22, 2019in Canadian Journal of Physiology and Pharmacology2.04
Marko Djuric2
Estimated H-index: 2
,
Tamara Nikolic3
Estimated H-index: 3
(University of Kragujevac)
+ 10 AuthorsVladimir Zivkovic8
Estimated H-index: 8
It has been assumed that the cardioprotective effects of propofol are due to its non-anaesthetic pleiotropic cardiac and vasodilator effects, in which gasotransmitters (NO, H2S and CO) as well as c...
This narrative review discusses the incidence, risk factors, mechanisms of injury, complications, and treatment regimens for accidental intra-arterial injection of medications. Despite awareness of the issue and the establishment of safety recommendations by national agencies, accidental iatrogenic intra-arterial injection of medications continues to occur. Most of these injuries are caused by accidental injection into an established arterial cannula or the inadvertent and unrecognized cannulati...
Published on Aug 1, 2018in European Journal of Pharmacology3.17
Liangqing Zhang6
Estimated H-index: 6
,
Zhihua Ruan1
Estimated H-index: 1
(HUM: Hubei University of Medicine)
+ 7 AuthorsZhe Hu5
Estimated H-index: 5
Abstract Ischemia-reperfusion injury(IRI), described as tissue damage caused by reversible ischemic injury or hypoxia prior to the blood supply restoration, is a common pathological phenomenon. In recent study, a hypoxia-reoxygenation (H/R) in the presence or absence of propofol posthypoxia treatment (P-PostH) cell model was built to simulate the condition of IRI, and researchers found propofol may protect cells by suppressing autophagic cell death. To investigate the mechanism underling the pro...
Published on Feb 1, 2018in Free Radical Biology and Medicine5.66
Gustavo H. Oliveira-Paula9
Estimated H-index: 9
(USP: University of São Paulo),
Lucas C. Pinheiro11
Estimated H-index: 11
(USP: University of São Paulo)
+ 4 AuthorsJosé Eduardo Tanus-Santos27
Estimated H-index: 27
(USP: University of São Paulo)
Abstract Propofol anesthesia is usually accompanied by hypotension. Studies have shown that the hypotensive effects of propofol increase in patients treated with angiotensin-converting enzyme inhibitors (ACEi). Given that both propofol and ACEi affect nitric oxide (NO) signaling, the present study tested the hypothesis that ACEi treatment induces pronounced hypotensive responses to propofol by increasing NO bioavailability. In this study we evaluated 65 patients, divided into three groups: hyper...
Marzena Wojewodzka-Zelezniakowicz5
Estimated H-index: 5
(UMB: Medical University of Białystok),
Anna Gromotowicz-Poplawska4
Estimated H-index: 4
(UMB: Medical University of Białystok)
+ 4 AuthorsEwa Chabielska16
Estimated H-index: 16
(UMB: Medical University of Białystok)
Introduction:The aim of this study was to investigate the effects of plasma and tissue angiotensin-converting enzyme inhibitors (ACE-Is) against propofol-induced endothelial dysfunction and to elucidate the involved mechanisms in vitro.Materials and methods:We examined the effects of propofol (50 μM), quinaprilat and enalaprilat (10−5 M) on fibrinolysis (t-PA, PAI-1, TAFI antigen levels), oxidative stress parameters (H2O2 and MDA antigen levels and SOD and NADPH oxidase mRNA levels) and nitric o...
Published on Apr 1, 2016in Life Sciences3.45
Wenguo Fan9
Estimated H-index: 9
(SYSU: Sun Yat-sen University),
Qin Liu2
Estimated H-index: 2
+ 4 AuthorsHongwen He10
Estimated H-index: 10
Nitric oxide (NO) is a free radical gas in the biological system, which is produced by nitric oxide synthase (NOS) family. NO acts as a biological mediator and plays important roles in different systems in humans. The NO/NOS system exerts a broad spectrum of signaling functions involved in vasodilation, inflammation, oxidative stress, cardioprotection and neuroprotection. It has been demonstrated that intravenous and volatile anesthetics (such as propofol, ketamine, midazolam, isoflurane, sevofl...
Published on Mar 8, 2016in Inflammation2.94
Xiaoyan Wang2
Estimated H-index: 2
(SDU: Shandong University),
Chengxiao Liu1
Estimated H-index: 1
(SDU: Shandong University),
Gongming Wang1
Estimated H-index: 1
(SDU: Shandong University)
High-mobility group box 1 (HMGB1) plays a key role in the development of acute lung injury (ALI). Propofol, a general anesthetic with anti-inflammatory properties, has been suggested to be able to modulate lipopolysaccharide (LPS)-induced ALI. In this study, we investigated the effects of propofol on the expression of HMGB1 in a rat model of LPS-induced ALI. Rats underwent intraperitoneal injection of LPS to mimic sepsis-induced ALI. Propofol bolus (1, 5, or 10 mg/kg) was infused continuously 30...
Published on Feb 22, 2016
Xiaowen Mao (WMU: Wenzhou Medical College), Zhi-Dong Ge22
Estimated H-index: 22
(WMU: Wenzhou Medical College)
+ 2 AuthorsZhengyuan Xia32
Estimated H-index: 32
(Li Ka Shing Faculty of Medicine, University of Hong Kong)
Reperfusion after prolonged ischemia is necessary to rescue injured hearts while it paradoxically worsens cardiac injury, a phenomenon termed myocardial ischemia-reperfusion injury (MIRI). Overproduction of reactive oxygen species, calcium overload, and inflammation contribute to the pathogenesis of MIRI. Propofol, a commonly used anesthetic with antioxidant property, has been shown to be cardioprotective in experimental studies and in some small clinical trials of cardiac surgery using cardiopu...