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Sensor materials for the detection of human neutrophil elastase and cathepsin G activity in wound fluid

Published on Jun 1, 2011in Experimental Dermatology 2.61
· DOI :10.1111/j.1600-0625.2011.01256.x
Andrea Hasmann8
Estimated H-index: 8
(Graz University of Technology),
Ulrike Gewessler4
Estimated H-index: 4
+ 8 AuthorsEva Wehrschuetz-Sigl7
Estimated H-index: 7
Abstract
Human neutrophil elastase (HNE) and cathepsin G (CatG) are involved in the pathogenesis of a number of inflammatory disorders. These serine proteinases are released by neutrophils and monocytes in case of infection. Wound infection is a severe complication regarding wound healing causing diagnostic and therapeutic problems. In this study we have shown the potential of HNE and CatG to be used as markers for early detection of infection. Significant differences in HNE and CatG levels in infected and non-infected wound fluids were observed. Peptide substrates for these two enzymes were successfully immobilised on different surfaces, including collagen, modified collagen, polyamide polyesters and silica gel. HNE and CatG activities were monitored directly in wound fluid via hydrolysis of the chromogenic substrates. Infected wound fluids led to significant higher substrate hydrolysis compared with non-infected ones. These different approaches could be used for the development of devices which are able to detect elevated enzyme activities before manifestation of infection directly on bandages. This would allow a timely intervention by medical doctors thus preventing severe infections.
  • References (69)
  • Citations (31)
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References69
Newest
Published on Jan 19, 2010in Experimental Dermatology 2.61
Stefanie Dressel4
Estimated H-index: 4
,
Jürgen Harder41
Estimated H-index: 41
+ 4 AuthorsRegine Gläser29
Estimated H-index: 29
Please cite this paper as: Differential expression of antimicrobial peptides in margins of chronic wounds. Experimental Dermatology 2010; 19: 628–632. Abstract: Skin wounds usually heal without major infections, although the loss of the mechanical epithelial barrier exposes the tissue to various bacteria. One reason may be the expression of antimicrobial peptides (AMP) of which some [human β-defensins (hBD) and LL-37] were recently shown to support additionally certain steps of wound healing. Th...
50 Citations Source Cite
Published on Sep 1, 2009in Electrochimica Acta 5.12
Dzaraini Kamarun3
Estimated H-index: 3
(Universiti Teknologi MARA),
Xinwei Zheng2
Estimated H-index: 2
(Queen Mary University of London)
+ 2 AuthorsSteffi Krause18
Estimated H-index: 18
(Queen Mary University of London)
Abstract Polyacrylamide hydrogel films cross-linked with the peptide sequence AAPVAAK were synthesised on piezoelectric quartz crystals. Degradation of the hydrogel films was monitored using a combination of quartz crystal microbalance (QCM) and electrochemical impedance measurements. The films were shown to degrade in the presence of human neutrophil elastase (HNE). The rate of degradation was directly related to the enzyme activity making the system suitable for the detection of HNE in the act...
18 Citations Source Cite
Published on Sep 1, 2009in Journal of Biotechnology 2.53
Anita Eberl9
Estimated H-index: 9
(Graz University of Technology),
Sonja Heumann14
Estimated H-index: 14
(Graz University of Technology)
+ 5 AuthorsGeorg Gübitz58
Estimated H-index: 58
(Graz University of Technology)
A lipase from Thermomyces lanuginosus and cutinases from Thermobifida fusca and Fusarium solani hydrolysed poly(ethylene terephthalate) (PET) fabrics and films and bis(benzoyloxyethyl) terephthalate (3PET) endo-wise as shown by MALDI-Tof-MS, LC–UVD/MS, cationic dyeing and XPS analysis. Due to interfacial activation of the lipase in the presence of Triton X-100, a seven-fold increase of hydrolysis products released from 3PET was measured. In the presence of the plasticizer N,N-diethyl-2-phenylace...
74 Citations Source Cite
Published on Mar 1, 2009in Biotechnology and Bioengineering 3.95
Sonja Heumann14
Estimated H-index: 14
,
Anita Eberl9
Estimated H-index: 9
+ 5 AuthorsGeorg Gübitz58
Estimated H-index: 58
(Graz University of Technology)
An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica . The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water inso- luble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. Treatment of polyamide 6 with this amidase led to an increased hydro- philicity based on rising height and tensiometr...
33 Citations Source Cite
Published on Oct 1, 2008in Journal of Polymer Science Part A 2.59
Tina Brueckner1
Estimated H-index: 1
(Graz University of Technology),
Anita Eberl9
Estimated H-index: 9
(Graz University of Technology)
+ 2 AuthorsGeorg Gübitz58
Estimated H-index: 58
(Graz University of Technology)
Alkaline and enzymatic hydrolyzes of poly(ethylene terephthalate) fabrics (PET) were mechanistically compared based on released degradation products (HPLC-UV-RI) and changes in surface properties [hydrophilicity, cationic dyeing, X-ray photoelectron spectroscopy (XPS)]. Enzymatic hydrolysis led to an increase in the amount of hydroxyl and carboxyl groups on the surface resulting in an enhanced water absorption and dyeability. Enzymes partially adsorbed to PET fabrics during hydrolysis were compl...
70 Citations Source Cite
Published on Aug 1, 2008in Sensor Letters
J. Vincent Edwards11
Estimated H-index: 11
,
Sonya Caston-Pierre2
Estimated H-index: 2
+ 2 AuthorsJudy Arnold2
Estimated H-index: 2
10 Citations Source Cite
Dorne R. Yager24
Estimated H-index: 24
(VCU Medical Center),
Robert A. Kulina1
Estimated H-index: 1
,
Laura A. Gilman1
Estimated H-index: 1
Wound healing of the skin is a complex biologic process involving temporal interactions between numerous types of cells, extracellular matrix molecules, and soluble factors. The process of repair can be viewed as involving 3 or 4 phases: homeostasis, inflammation, synthesis, and remodeling. These phases occur at different times and differ in their cellular, biochemical, and physiologic requirements. Disruption of one or more of these interactions can significantly interfere with the repair proce...
68 Citations Source Cite
Sonja Heumann14
Estimated H-index: 14
(Graz University of Technology),
Anita Eberl9
Estimated H-index: 9
+ 5 AuthorsGeorg Gübitz58
Estimated H-index: 58
(Graz University of Technology)
Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoyloxyethyl) terephthalate and adipic acid bishexyl-amide, were also capable of increasi...
87 Citations Source Cite
Published on Nov 1, 2005in Biomaterials 8.81
Ute Schönfelder3
Estimated H-index: 3
(Schiller International University),
Martin Abel8
Estimated H-index: 8
+ 3 AuthorsUta-Christina Hipler28
Estimated H-index: 28
(Schiller International University)
Exudates from non-healing wounds contain elevated levels of proteolytic enzymes, like elastase from polymorphonuclear granulocytes (PMN elastase), reactive oxygen species (ROS) and reactive nitrogen species (RNS). The overproduction of proteolytic enzymes leads to reduced concentrations of growth factors and proteinase inhibitors, resulting in an imbalance between degradation and remodelling processes. Thus, the reduction of protein-degrading enzymes and scavenging of ROS and RNS seem to be suit...
69 Citations Source Cite
Published on Oct 1, 2005in Journal of Peptide Research
J.V. Edwards2
Estimated H-index: 2
(United States Department of Agriculture),
Sonya Caston-Pierre2
Estimated H-index: 2
(United States Department of Agriculture)
+ 1 AuthorsW. Goynes1
Estimated H-index: 1
(United States Department of Agriculture)
Abstract: An assessment of elastase-substrate kinetics and adsorption at the solid–liquid interface of peptide-bound resin was made in an approach to the solid-phase detection of human neutrophil elastase (HNE), which is found in high concentration in chronic wound fluid. N-succinyl-alanine-alanine-proline-valine-p-nitroanilide (suc-Ala-Ala-Pro-Val-pNA), a chromogenic HNE substrate, was attached to glycine-cross-linked ethoxylate acrylate resins (Gly-CLEAR) by a carbodiimide reaction. To assess ...
12 Citations Source Cite
Cited By31
Newest
Published on Jun 1, 2019in Biotechnology Reports
Ana Vanessa Fernandes Ferreira2
Estimated H-index: 2
(University of Minho),
Egipto Antunes2
Estimated H-index: 2
(University of Minho)
+ 4 AuthorsArtur Cavaco Paulo48
Estimated H-index: 48
(University of Minho)
Abstract Recent studies have demonstrated that human neutrophil elastase (HNE) can be used as marker for inflammation/infection of chronic wounds since it was found to be present in high concentration in exudate collected from chronic wounds. Biosensors used in wound care benefit from a chromogenic signalling due to the readiness of signal interpretation, but the most common use faint yellow chromogenic molecules such as p -nitroaniline ( p Na). In addition, if to be converted into smart dressin...
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Published on Dec 1, 2018in Trends in Biotechnology 13.58
Hossein Derakhshandeh2
Estimated H-index: 2
(University of Nebraska–Lincoln),
Sara Saheb Kashaf1
Estimated H-index: 1
(University of Chicago)
+ 2 AuthorsAli Tamayol3
Estimated H-index: 3
(University of Nebraska–Lincoln)
Chronic non-healing wounds are major healthcare challenges that affect a noticeable number of people; they exert a severe financial burden and are the leading cause of limb amputation. Although chronic wounds are locked in a persisting inflamed state, they are dynamic and proper therapy requires identifying abnormalities, administering proper drugs and growth factors, and modulating the conditions of the environment. In this review article, we discuss technologies that have been developed to act...
4 Citations Source Cite
Published on Feb 1, 2018in Diabetic Medicine 3.13
John R. Ingram15
Estimated H-index: 15
(Cardiff University),
S. Cawley1
Estimated H-index: 1
(Cardiff and Vale University Health Board)
+ 8 AuthorsKerenza Hood46
Estimated H-index: 46
(Cardiff University)
Aims Deciding if a diabetic foot ulcer is infected in a community setting is challenging without validated point-of-care tests. Four inflammatory biomarkers were investigated to develop a composite algorithm for mildly infected diabetic foot ulcers: venous white cell count, C-reactive protein (CRP) and procalcitonin, and a novel wound exudate calprotectin assay. Calprotectin is a marker of neutrophilic inflammation. Methods In a prospective study, people with uninfected or mildly infected diabet...
1 Citations Source Cite
Published on Dec 1, 2017in Journal of Chromatography A 3.72
Syntia Fayad4
Estimated H-index: 4
(University of Orléans),
Philippe Morin20
Estimated H-index: 20
(University of Orléans),
Reine Nehmé11
Estimated H-index: 11
(University of Orléans)
Abstract Elastase, collagenase, hyaluronidase and tyrosinase, are very interesting enzymes due to their direct implication in skin aging and as therapeutic hits. Different techniques can be used to study these enzymes and to evaluate the influence of effectors on their kinetics. Nowadays, analytical techniques have become frequently used tools for miniaturizing enzyme assays. The main intention of this article is to review chromatographic and electrophoretic tools that study the four enzymes abo...
4 Citations Source Cite
Published on Oct 30, 2017in Cochrane Database of Systematic Reviews 6.75
Maggie J Westby4
Estimated H-index: 4
,
Jo C Dumville36
Estimated H-index: 36
+ 4 AuthorsRichard D Riley37
Estimated H-index: 37
(Keele University)
This is a protocol for a Cochrane Review (Prognosis). The objectives are as follows: To determine whether protease activity is an independent prognostic factor for the healing of venous leg ulcers.
1 Citations Source Cite
Published on Mar 2, 2017
Kristin M. Priebatsch2
Estimated H-index: 2
,
Marc Kvansakul22
Estimated H-index: 22
+ 1 AuthorsMark D. Hulett33
Estimated H-index: 33
Divalent metal ions are essential nutrients for all living organisms and are commonly protein-bound where they perform important roles in protein structure and function. This regulatory control from metals is observed in the relatively abundant plasma protein histidine-rich glycoprotein (HRG), which displays preferential binding to the second most abundant transition element in human systems, Zinc (Zn2+). HRG has been proposed to interact with a large number of protein ligands and has been impli...
7 Citations Source Cite
Published on Feb 1, 2017in Applied Microbiology and Biotechnology 3.34
Ana Vanessa Fernandes Ferreira2
Estimated H-index: 2
(University of Minho),
Ilana Perelshtein20
Estimated H-index: 20
(Bar-Ilan University)
+ 3 AuthorsArtur Cavaco Paulo48
Estimated H-index: 48
(University of Minho)
Chronic wound fluids have elevated concentration of human neutrophil elastase (HNE) which can be used as inflammation/infection marker. Our goal is to develop functional materials for fast diagnosis of wound inflammation/infection by using HNE as a specific marker. For that, fluorogenic peptides with a HNE-specific cleavage sequence were incorporated into traditional textile dressings, to allow real-time detection of the wound status. Two different fluorogenic approaches were studied in terms of...
3 Citations Source Cite
Published on Dec 1, 2016in Archives of Oral Biology 2.05
Idil Kurtulus Waschulewski1
Estimated H-index: 1
,
Aslan Gökbuget6
Estimated H-index: 6
(Istanbul University)
+ 4 AuthorsWerner Götz27
Estimated H-index: 27
Abstract Objective Type I plasminogen deficiency (Plgdef) is an uncommon chronic inflammation of mucous membranes. Gingival enlargements usually proceed with progressive periodontal destruction and tooth-loss. Plasmin(ogen)-independent enzymatic mechanisms for fibrin clearance have already been discussed in the literature. Our primary objective was to verify, immunohistochemically, the occurrence of different enzymatic factors involved in tissue breakdown of inflamed compared to healthy gingiva....
3 Citations Source Cite
Published on Aug 1, 2016in European Polymer Journal 3.74
Mir-Morteza Sadat Ebrahimi3
Estimated H-index: 3
(University of Siegen),
Nina Dohm2
Estimated H-index: 2
(University of Mainz)
+ 2 AuthorsHolger Schönherr37
Estimated H-index: 37
(University of Siegen)
Abstract We report on the development of an autonomously reporting indicator hydrogel for the rapid and selective detection of enzymes secreted by the food-borne biosafety level 3 pathogen enterohemorrhagic Escherichia coli ( E. coli ) O157:H7 (EHEC) and the differentiation of this pathogen from the non-virulent E. coli strain K12. To introduce the sensing functionality, chitosan hydrogel films were equipped with fluorogenic substrates for the detection of α-galactosidase (α-Gal) and β-galactosi...
2 Citations Source Cite
Published on Jan 1, 2016
James Davis30
Estimated H-index: 30
(Ulster University),
A. McLister (Ulster University)
The inevitable delay in wound healing associated with a chronic wound renders the patient much more susceptible to wound infection. It has long been recognized that the early identification of infection can significantly improve patient outcome and can help prevent the development of life-threatening complications. At present, there remains considerable reliance on subjective parameters, and while there is a wealth of sophisticated detection techniques that can aid identification of individual s...
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