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Vibrio hippocampi sp. nov., a new species isolated from wild seahorses (Hippocampus guttulatus).
Abstract
A Gram-negative, facultatively anaerobic, motile and slightly curved rod-shaped bacterium (BFLP-4T) was isolated from the faeces of wild seahorses ( Hippocampus guttulatus ) captured in northwest Spain (Toralla, Galicia). Strain BFLP-4T grew at 10–35 °C and pH 5–9 (optimally at 20 °C and pH 7.2) and at salt concentrations in the range 0–7% w/v NaCl. The G+C content of the DNA was 49.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BFLP-4T was a member of the genus Vibrio , being most closely related to Vibrio ichthyoenteri (97.1%), Vibrio mediterranei (96.7%), Vibrio scophthalmi (96.7%) and Vibrio sinaloensis (96.6%). A phylogenetic analysis based on recA gene sequences also supported the affiliation of strain BFLP-4T to the genus Vibrio . Strain BFLP-4T could be readily differentiated from other closely related species by several phenotypic properties and fatty acid profiles. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-4T represents a novel species within the genus Vibrio , for which the name Vibrio hippocampi sp. nov. is proposed. The type strain is BFLP-4T (=DSM 22717T=LMG 25354T).

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  • References (19)
  • Cited By (7)
1968 in Methods in Enzymology [IF: 1.98]
M. Mandel1
Estimated H-index: 1
,
J. Marmur1
Estimated H-index: 1
Abstract The GC content of highly purified DNA samples can be estimated from their thermal denaturation temperatures, employing small amounts of DNA (10–40 μg). The method is simple, convenient and utilizes readily available laboratory equipment; the thermal denaturation profile also gives a good picture of the quality of the DNA preparation. Denatured DNA present in native preparations, or single stranded DNA obtained by denaturing native DNA, or isolated from natural sources such as bacterioph...
806 Citations Source Cite
J. J. Farmer1
Estimated H-index: 1
37 Citations Cite
J D Buck1
Estimated H-index: 1
A rapid nonstaining (KOH) method for the determination of the Gram reactions of bacteria is described, and its application to marine isolates is discussed. All gram-positive and gram-negative results obtained by Gram staining were confirmed by the KOH method. Gram-variable bacteria produced equivocal results.
636 Citations Download PDF
2008 in Research in Microbiology [IF: 2.37]
Etinosa O. Igbinosa14
Estimated H-index: 14
(University of Fort Hare),
Anthony I. Okoh29
Estimated H-index: 29
(University of Fort Hare)
Discharge of inadequately treated sewage effluents into the environment in developing countries has increased over the years, leading to deterioration of water quality of major watersheds in developing nations and consequently an increased incidence of emerging pathogens such as Vibrio species, the prevalence of which has been generally underestimated in developing nations. This review underscores the need for a proactive approach to risk factors for emerging Vibrio infections, so as to establis...
81 Citations Source Cite
I. Noguerola1
Estimated H-index: 1
(University of Barcelona),
Anicet R. Blanch35
Estimated H-index: 35
(University of Barcelona)
Aims: To define a binary biochemical key for the identification of all recognized Vibrio spp. Methods and Results: A matrix of phenotypical results was developed based on the previous taxonomical studies and the first description manuscripts. A unification of results from various sources was also performed to integrate different taxonomical studies within the same data matrix. Established criteria for selecting the optimal set of tests yielded the highest discrimination, as well as the lowest nu...
96 Citations Source Cite
2007 in Food Microbiology [IF: 4.09]
Juan José Rodríguez Herrera15
Estimated H-index: 15
(Spanish National Research Council),
Marta López Cabo16
Estimated H-index: 16
(Spanish National Research Council),
Ángel F. González27
Estimated H-index: 27
(Spanish National Research Council)
... more
Abstract The kinetics of adhesion of five Staphylococcus aureus subsp. aureus strains (CECT 976, 4459, 4465, 4466 and 5191) to polypropylene at 25 °C in the absence of nutrients (PBS medium) were initially compared. Those strains with the highest (CECT 4459) and the lowest (CECT 976) adhesion levels were selected for further studying the effects of a nutrient-rich adhesion-promoting medium (TSB plus 1% glucose—TSBG) as well as of a conditioning film consisting of dried mussel cooking juices (MCJ...
71 Citations Source Cite
Giuseppina Tantillo17
Estimated H-index: 17
,
M. Fontanarosa1
Estimated H-index: 1
,
A. Di Pinto8
Estimated H-index: 8
... more
71 Citations Source Cite
1990 in Journal of Molecular Biology [IF: 4.89]
Stephen F. Altschul45
Estimated H-index: 45
(National Institutes of Health),
Warren Gish15
Estimated H-index: 15
(National Institutes of Health),
Webb Miller88
Estimated H-index: 88
(Pennsylvania State University)
... more
A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score. Recent mathematical results on the stochastic properties of MSP scores allow an analysis of the performance of this method as well as the statistical significance of alignments it generates. The basic algorithm is simple and robust; it can be implemented in a number of ways and applied in a vari...
57.7k Citations Download PDF Cite
José Luis Balcázar32
Estimated H-index: 32
(Spanish National Research Council),
Alfonso Gallo-Bueno1
Estimated H-index: 1
(Spanish National Research Council),
M. Planas16
Estimated H-index: 16
(Spanish National Research Council)
... more
Vibrio species isolated from diseased seahorses were characterized by PCR amplification of repetitive bacterial DNA elements (rep-PCR) and identified by 16S ribosomal RNA gene sequence analysis. The results demonstrated that Vibrio alginolyticus and Vibrio splendidus were predominant in the lesions of these seahorses. To our knowledge, this is the first time that these bacterial species have been associated with disease symptoms in captive-bred seahorses.
41 Citations Source Cite
Jonathan A. Eisen79
Estimated H-index: 79
(Stanford University)
The evolution of the RecA protein was analyzed using molecular phylogenetic techniques. Phylogenetic trees of all currently available complete RecA proteins were inferred using multiple maximum parsimony and distance matrix methods. Comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. The RecA trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the α, β, γ...
212 Citations Download PDF Cite
  • References (19)
  • Cited By (7)
2014 in Current Microbiology [IF: 1.37]
Ana Paula B. Moreira10
Estimated H-index: 10
(Federal University of Rio de Janeiro),
Gwen Duytschaever6
Estimated H-index: 6
(Federal University of Rio de Janeiro),
Luciane A. Chimetto Tonon5
Estimated H-index: 5
(Federal University of Rio de Janeiro)
... more
Three novel isolates (A-354T, A-328, and A-384) were retrieved from apparently healthy scleractinian Madracis decactis in the remote St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil. The novel isolates formed a distinct lineage based on the phylogenetic reconstruction using the 16S rRNA and pyrH gene sequences. They fell into the Mediterranei clade and their closest phylogenetic neighbour was V. mediterranei species, sharing upto 98.1 % 16S rRNA gene sequence similarity. Genomic analys...
9 Citations Download PDF Cite
Feng Li2
Estimated H-index: 2
(Chinese Academy of Sciences),
Kai Wang2
Estimated H-index: 2
(Ludong University),
Wei Luo1
Estimated H-index: 1
(Chinese Academy of Sciences)
... more
This investigation examined the intestinal microbial flora among healthy and intestinal-diseased seahorses Hippocampus trimaculatus, Hippocampus erectus, and Hippocampus spinosissimus by utilizing polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) and densitometric analysis. Results demonstrated that 16 disparate DGGE bands belong to six major bacterial groups, which were Vibrionace, Enterobacteriacea, Rhodobacteraceae, Sphingomonadaceae, Flavobacteriaceae, and Alcaligenace...
5 Citations Source Cite
2012 in Aquaculture Research [IF: 1.48]
Tanu1
Estimated H-index: 1
(Council for Scientific and Industrial Research),
Deepti D. Deobagkar14
Estimated H-index: 14
(Savitribai Phule Pune University),
Rakhee Khandeparker1
Estimated H-index: 1
(Council for Scientific and Industrial Research)
... more
The microenvironment of bacteria associated with the gastrointestinal tract of an animal influences the host in many ways, including the metabolism of several nutrients. Isolation, molecular characterisation and enzymatic activities of culturable bacteria associated with the gastrointestinal tract of hatcheryreared oceanic yellow seahorse, Hippocampus kuda, were investigated in the present study. 16S rRNA sequencing of bacterial isolates yielded 10 different bacterial genera belonging to two maj...
20 Citations Source Cite
Eletherios Chalkiadakis2
Estimated H-index: 2
(IFREMER),
R. Dufourcq2
Estimated H-index: 2
(Pasteur Institute),
Sophie Schmitt4
Estimated H-index: 4
(IFREMER)
... more
Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identif...
9 Citations Download PDF Cite
Fl Thompson16
Estimated H-index: 16
,
Margo Cnockaert22
Estimated H-index: 22
,
P. De Vos ... more
Three novel isolates (A-354 T , A-328, and A-384) were retrieved from apparently healthy scleractinian Madra- cis decactis in the remote St Peter & St Paul Archipelago, Mid- Atlantic Ridge, Brazil. The novel isolates formed a distinct lineage based on the phylogenetic reconstruction using the 16S rRNA and pyrH gene sequences. They fell into the Mediterranei clade and their closest phylogenetic neighbour was V. mediterranei species, sharing upto 98.1 % 16S rRNA gene sequence similarity. Genomic a...
Kai Wang2
Estimated H-index: 2
(Ludong University),
Yanhong Zhang6
Estimated H-index: 6
(Chinese Academy of Sciences),
Feng Li2
Estimated H-index: 2
(Chinese Academy of Sciences)
... more
[Wang, Kai] Ludong Univ, Sch Agr, Yantai 264025, Peoples R China; [Zhang, Yanhong; Li, Feng; Lin, Qiang] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
2 Citations Source Cite

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