Abstract A survey on perkinsosis was performed involving 15 locations scattered along the Galician coast (NW Spain) and four clam species with high market value ( Ruditapes decussatus , Ruditapes philippinarum , Venerupis corrugata and Polititapes rhomboides ). The prevalence of Perkinsus parasites was estimated by PCR using genus-specific primers. The highest percentage of PCR-positive cases for perkinsosis corresponded to clams R . decussatus and V . corrugata , while lower values were detecte...
(Spanish National Research Council), Miquel Planas13
Estimated H-index: 13
(Spanish National Research Council)... more
Breeding in captivity for research or exhibition (e.g. in aquaria) can replace the capture of wild specimens of endangered species and allow controlled reinforcement of wild populations. With this aim, diet analysis and establishing the adequate prey are determinant factors for breeding success. However, non-invasive approaches such as faecal DNA analysis are advisable for analysing the diet of these species. Therefore, the aim of the present study was to demonstrate the usefulness of faecal DNA...
(Spanish National Research Council), Álvaro Roura9
Estimated H-index: 9
(Spanish National Research Council)... more
The Scuticociliatia subclass includes hundreds of species with mostly uncertain phylogenetic relationships and classifications ( Alvarez-Pellitero et al. 2004). On the basis of morphological and morphogenetical data, Lynn (2008) recognized three Scuticociliatia orders: Philasterida, Thigmotrichida and Pleuronematida. They are endemic to worldwide coastal ecosystems where they thrive either as freeliving organisms or parasitic to aquatic animals. In fact, these facultative ciliated protists can o...
(Spanish National Research Council), Miquel Planas13
Estimated H-index: 13
(Spanish National Research Council), José Pintado15
Estimated H-index: 15
(Spanish National Research Council)
A Gram-positive, aerobic, non-motile, non-sporulating, acid-fast, and rod-shaped bacterium (BFLP-6T), previously isolated from a seahorse (Hippocampus guttulatus) with tail rot, was studied using a polyphasic taxonomic approach. Growth occurred at 15–35 °C (optimum 25 °C), at pH 5.0–10.0 (optimum pH 7.0) and at NaCl concentrations between 0 and 6 % (w/v). The G+C content of DNA was 66.7 mol%. The predominant fatty acids were C18:1 ω9c, C16:0 and C16:1 ω6c. A mycolic acid pattern of alpha-mycolat...
Growth, biofilm formation, antagonism and residence time in green seawater tanks maintained under fish rearing conditions of Phaeobacter 27-4 were studied in commercial biofilters made from plastic, sintered glass and ceramic. Phaeobacter reached 108–109 CFU cm−3 and formed rosettes in all materials, but a multilayer biofilm was only observed in the ceramic biofilters. In sterile seawater, plastic and ceramic biofilters reduced Vibrio anguillarum and V. splendidus concentration in one-two Log af...
The rearing environment of first-feeding turbot larvae, usually with high larvae densities and organic matter concentrations, may promote the growth of opportunistic pathogenic Vibrionaceae bacteria, compromising the survival of the larvae. The aim of this study was to assess the effectiveness of the biofilm-forming probiotic Phaeobacter 27-4 strain grown on a ceramic biofilter (probiofilter) in preventing Vibrio anguillarum infections in turbot larvae. In seawater with added microalgae and main...
Abstract The effect of temperature (15, 18 and 21 °C) on the early development of the long-snouted seahorse Hippocampus guttulatus were analysed, both under feeding (until day 30 post partum) and starving conditions. Fed and starving seahorses maintained at 15 °C grew at suboptimal conditions. At this temperature, mortalities started earlier and the swimming activity was reduced. In fed juveniles, final survivals (21.1, 85.9 and 81.1% at 15, 18 and 21 °C, respectively) and wet body mass (14.7, 5...
Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular iden...