Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods

In hydrogels of cross-linked polysaccharides, the total amount of cross-linker and the degree of cross-linking influence the properties of the hydrogel. The substitution position of the cross-linker on the polysaccharide is another parameter that can influence hydrogel properties; hence methods for detailed structural analysis of the substitution pattern are required. NMR and LC–MS methods were developed to determine the positions and amounts of...