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Vascular endothelial growth factor mediates angiogenic activity during the proliferative phase of wound healing

Published on Jun 1, 1998in American Journal of Pathology 4.07
Nicholas N. Nissen2
Estimated H-index: 2
(Loyola University Chicago),
Peter J. Polverini63
Estimated H-index: 63
+ 3 AuthorsLuisa A. DiPietro43
Estimated H-index: 43
Angiogenesis is an essential component of normal wound repair, yet the primary mediators of wound angiogenesis have not been well described. The current study characterizes the contribution of vascular endothelial cell growth factor (VEGF) to the angiogenic environment of human surgical wounds. Surgical wound fluid samples (n = 70) were collected daily for up to 7 postoperative days (POD) from 14 patients undergoing mastectomy or neck dissection. VEGF levels in surgical wound fluid were lowest on POD 0, approximating values of serum, but increased steadily through POD 7. An opposite pattern was noted for basic fibroblast growth factor-2. Fibroblast growth factor-2, which has been previously described as a wound angiogenic factor, exhibited highest levels at POD 0, declining to near serum levels by POD 3. Surgical wound fluid form all time points stimulated marked endothelial cell chemotaxis and induced a brisk neovascular response in the rat corneal micropocket angiogenesis assay. Antibody neutralization of VEGF did not affect the in vitro chemotactic or the in vivo angiogenic activity early wound samples (POD 0). In contrast, VEGF neutralization significantly attenuated both chemotactic activity (mean decrease 76 +/- 13%, P < 0.01) and angiogenic activity (5 of 5 samples affected) of later wound samples (POD 3 and 6). The results suggest a model of wound angiogenesis in which an initial angiogenic stimulus is supplied by fibroblast growth factor-2, followed by a subsequent and more prolonged angiogenic stimulus mediated by VEGF.
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Published on Apr 11, 2019in Nature Reviews Molecular Cell Biology 35.61
Jiajun Zhu1
Estimated H-index: 1
(Memorial Sloan Kettering Cancer Center),
Craig B. Thompson157
Estimated H-index: 157
(Memorial Sloan Kettering Cancer Center)
Cellular metabolism is at the foundation of all biological activities. The catabolic processes that support cellular bioenergetics and survival have been well studied. By contrast, how cells alter their metabolism to support anabolic biomass accumulation is less well understood. During the commitment to cell proliferation, extensive metabolic rewiring must occur in order for cells to acquire sufficient nutrients such as glucose, amino acids, lipids and nucleotides, which are necessary to support...
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Published on Feb 1, 2019in The FASEB Journal 5.59
Takuro Miyazaki11
Estimated H-index: 11
(Showa University),
Shogo Haraguchi9
Estimated H-index: 9
(Showa University)
+ 1 AuthorsAkira Miyazaki37
Estimated H-index: 37
(Showa University)
The transformation of fibroblasts to myofibroblasts plays a major role in fibrogenic responses during dermal wound healing. We show a contribution of calpain systems (intracellular regulatory protease systems) in vascular endothelial cells (ECs) to myofibroblast differentiation in wound sites. Dermal wound healing experiments in mice found that calpastatin (an endogenous inhibitor of calpains) is enriched in preexisting vessels but not in newly formed capillaries. Transgenic overexpression of ca...
1 Citations Source Cite
Published on Jan 16, 2019in PLOS ONE 2.77
Katharina Sommer3
Estimated H-index: 3
Heike Jakob9
Estimated H-index: 9
+ 4 AuthorsAnna L. Sander11
Estimated H-index: 11
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Published on Jan 1, 2019in Biomaterials Science 5.83
Yan Zhang1
Estimated H-index: 1
(Nanjing University),
Keyu Tang (Sun Yat-sen University)+ 7 AuthorsWeiling He (Sun Yat-sen University)
Nitric oxide (NO) plays a pivotal role in the cutaneous healing process and a topical supplement of NO is beneficial for wound repair. In this work, a novel polyethylenimine (PEI) based diazeniumdiolate nitric oxide donor was prepared. The obtained polymer (PEI-PO-NONOate) was characterized by Fourier transform infrared (FTIR) spectroscopy, UV-vis absorption spectra, and nuclear magnetic resonance (NMR). The PEI-PO-NONOate polymer was stable under anhydrous conditions at different temperatures. ...
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Published on Dec 1, 2018in Journal of Molecular Medicine
Tsung Lin Cheng7
Estimated H-index: 7
(Kaohsiung Medical University),
Po Ku Chen7
Estimated H-index: 7
(National Cheng Kung University)
+ 6 AuthorsChao-Han Lai10
Estimated H-index: 10
(National Cheng Kung University)
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Published on Nov 29, 2018in Clinical Oral Investigations 2.39
Thanasak Rakmanee5
Estimated H-index: 5
(Thammasat University),
Elena Calciolari6
Estimated H-index: 6
(Queen Mary University of London)
+ 4 AuthorsNikos Donos47
Estimated H-index: 47
(Queen Mary University of London)
Objectives To describe changes in growth factor mediators in the gingival crevicular fluid (GCF) of patients with aggressive periodontitis (AgP) undergoing regenerative (GTR) and access flap (AF) surgery.
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Published on Oct 3, 2018in Expert Review of Anticancer Therapy 2.35
Li Li1
Estimated H-index: 1
(Third Military Medical University),
Henry Mok1
Estimated H-index: 1
(Baylor College of Medicine)
+ 5 AuthorsYohannes T. Ghebre4
Estimated H-index: 4
(Baylor College of Medicine)
ABSTRACTIntroduction: Chemotherapy and radiation therapy are two mainstream strategies applied in the treatment of cancer that is not operable. Patients with hematological or solid tumor malignancies substantially benefit from chemotherapeutic drugs and/or ionizing radiation delivered to the site of malignancy. However, considerable adverse effects, including lung inflammation and fibrosis, are associated with the use of these treatment modalities.Areas covered: As we move toward the era of prec...
2 Citations Source Cite
Published on Sep 1, 2018in Advanced Drug Delivery Reviews 13.66
Austin Veith1
Estimated H-index: 1
(University of Texas at Austin),
Kayla Henderson2
Estimated H-index: 2
(University of Texas at Austin)
+ 2 AuthorsAaron B. Baker20
Estimated H-index: 20
Abstract The enhancement of wound healing has been a goal of medical practitioners for thousands of years. The development of chronic, non-healing wounds is a persistent medical problem that drives patient morbidity and increases healthcare costs. A key aspect of many non-healing wounds is the reduced presence of vessel growth through the process of angiogenesis. This review surveys the creation of new treatments for healing cutaneous wounds through therapeutic angiogenesis. In particular, we di...
4 Citations Source Cite
Published on Sep 1, 2018in Clinical and Experimental Ophthalmology 3.22
Arezoo Miraftabi2
Estimated H-index: 2
(Iran University of Medical Sciences),
Naveed Nilforushan8
Estimated H-index: 8
(Iran University of Medical Sciences)
+ 3 AuthorsMaryam Yadgari1
Estimated H-index: 1
(Iran University of Medical Sciences)
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Lei Hong (Ningbo University), Meiting Shen (Ningbo University)+ 4 AuthorsYabin Zhu9
Estimated H-index: 9
(Ningbo University)
In this work, two kinds of hyaluronic acid (HA)-based hydrogels were fabricated: one is made from physical freezing-thawing of HA solution (HA1), and the other is from chemical cross-linking of HA and polysaccharide (HA2). They were applied to repair full-thickness skin defects with New Zealand rabbits as the test animals, using powder HA and cotton dress as the references. The wound starts to heal after wounds were disinfected with iodine followed by coating with HA2, HA1, HA and cotton dress (...
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