Review paper
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
Abstract
The sequence of small-subunit rRNA varies in an orderly manner across phylogenetic lines and contains segments that are conserved at the species, genus, or kingdom level. By directing oligonucleotide primers at sequences conserved throughout the eubacterial kingdom, we amplified bacterial 16S ribosomal DNA sequences with the polymerase chain reaction. Priming sites were located at the extreme 5' end, the extreme 3' end, and the center of 16S...
Paper Details
Title
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
Published Date
Sep 1, 1990
Volume
28
Issue
9
Pages
1942 - 1946
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