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Journal of Clinical Microbiology
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#1P. Lewis White (Cardiff University)H-Index: 20
#2Jessica S. Price (Cardiff University)H-Index: 2
Last. Matthijs Backx (Cardiff University)H-Index: 2
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Objectives Management of invasive aspergillosis has been improved by biomarker assays, but limited accessibility and batch testing limit impact. Lateral flow assays (LFA) are a simple method for use outside specialist centres, provided performance is acceptable. The objective of this study was to determine the performance of the recently released IMMY sona Aspergillus LFA when testing serum samples. Methods The study took the form of a retrospective, anonymous case/control study, comprising 179 ...
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The laboratory diagnosis of Lyme disease relies upon serologic testing. A standard or modified two-tiered algorithm is used to enhance the accuracy of antibody detection. However, this approach suffers from a lack of sensitivity in early Lyme disease. Ongoing efforts to develop more sensitive antibody detection technologies and other diagnostic approaches is dependent upon the availability of quality-assured biospecimens linked to reliable clinical data. In this issue of the Journal of Clinical ...
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#1Amin S. Asfor (Institute for Animal Health)H-Index: 5
#2N. Howe (Institute for Animal Health)
Last. Emiliana BrocchiH-Index: 22
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Diagnostic tests for foot-and-mouth disease (FMD) include the detection of antibodies against either the viral non-structural proteins or the capsid. The detection of antibodies against the structural proteins (SP) of the capsid can be used to monitor seroconversion in both infected and vaccinated animals. However, SP tests need to be tailored to the individual FMD virus serotype and their sensitivity performances may be affected by antigenic variability within each serotype and mismatching betw...
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#1S. Khan (Zhengzhou University)H-Index: 3
#1Suliman KhanH-Index: 7
Last. Mengzhou XueH-Index: 1
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The new decade of the 21 st century (2020) started with the emergence of novel coronavirus known as SARS-CoV-2 that caused an epidemic of coronavirus disease (COVID-19) in Wuhan, China. It is the third highly pathogenic and transmissible coronavirus after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in humans. The source of origin, transmission to humans and mechanisms associated with the pathogenicity of SARS-CoV-2 ...
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#1Thomas Z. Thompson (Georgia Regents University)H-Index: 3
#2Allison R. McMullen (Georgia Regents University)
Each year there are an estimated 11 million visits to ambulatory care centers for pharyngitis in children between the ages of 3-18 years old. While there are many causes of pediatric pharyngitis, group A streptococcal pharyngitis represents 15-30% of infections and is the only cause for which treatment is recommended. Unfortunately, clinical suspicion is insufficient for the accurate diagnosis of group A streptococcal pharyngitis and laboratory testing for confirmation of Streptococcus pyogenes ...
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Last. Yehuda Carmeli (TAU: Tel Aviv University)H-Index: 72
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The IR Biotyper is a new automated typing system based on Fourier-transform infrared (FT-IR) spectroscopy that gives results within 4 hours. We aimed: 1) to use the IR Biotyper to retrospectively analyze an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) in a neonatal intensive care unit and to compare results to BOX-PCR and whole genome sequencing (WGS) as the gold standard; and 2) to assess how the cut-off values used to define clusters affect the discrim...
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Background: Limited treatment options contribute to high morbidity/mortality rates with carbapenem-resistant, gram-negative bacterial infections. New approaches for carbapenemase-producing organism (CPO) detection may help inform clinician decision-making on patient treatment and infection control. BD Phoenix™ CPO detect (“CPO detect”) detects and classifies carbapenemases in Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa during susceptibility testing. The clinical perform...
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#1Cyril Chik-Yan YipH-Index: 9
#1Cyril Chik-Yan Yip (Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 3
Last. Kwok-Hung Chan (Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 10
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Accurate detection of influenza A virus (IAV) is crucial for patient management, infection control, and epidemiological surveillance. The World Health Organization and the Centers for Disease Control and Prevention have recommended using the M gene as the diagnostic gene target for reverse-transcription-PCR (RT-PCR). However, M gene RT-PCR has reduced sensitivity for recent IAV due to novel gene mutations. Here, we sought to identify novel diagnostic targets for IAV RT-PCR assay using long-read ...
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#2John W. Breneman (Bio-Rad Laboratories)H-Index: 5
Last. Ravi KaulH-Index: 8
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Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferi sensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in patients with early Lyme infection. Asymptomatic subjects from endemic and non-endemic origins demonstrated a seroreactivity rate of approximately 4% that was similar to other comme...
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#1Rania FrancisH-Index: 1
#2Maxime MioulaneH-Index: 6
Last. Bernard La ScolaH-Index: 60
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Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although current serological and molecular detection tools allow a reliable diagnosis of the disease, culture of C. burnetii strains is mandatory to assess their susceptibility to antibiotics and sequence their genome in order to optimize patient management and epidemiological studies. However, cultivating this fastidious microorg...
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