scinapse is loading now...

Development of an enhanced proteomic method to detect prognostic and diagnostic markers of healing in chronic wound fluid.

Published on Dec 7, 2007in British Journal of Dermatology 6.13
· DOI :10.1111/j.1365-2133.2007.08362.x
Melissa Laura Fernandez4
Estimated H-index: 4
(Queensland University of Technology),
James A. Broadbent7
Estimated H-index: 7
(Queensland University of Technology)
+ 2 AuthorsZee Upton31
Estimated H-index: 31
(Queensland University of Technology)
Abstract
Summary Background  Chronic venous leg ulcers are a significant cause of pain, immobility and decreased quality of life for patients with these wounds. In view of this, research efforts are focused on multiple factors in the wound environment to obtain information regarding the healing of ulcers. Objectives  Chronic wound fluid (CWF), containing a complex mixture of proteins, is an important modulator of the wound environment, and therefore we hypothesized that these proteins may be indicators of the status of wounds and their potential to heal or otherwise. To explore this we developed and validated a proteomic approach to analyse CWF. Methods  In this study, pooled CWF was depleted of high abundant proteins using immunoaffinity chromatography. The flow-through and bound fractions were collected, concentrated, desalted and analysed using a range of techniques. Each fraction was further separated using two-dimensional (2D) gel electrophoresis and 2D liquid chromatography and analysed using mass spectrometry (MS). Results  Western blot analysis against three high abundant proteins confirmed the selective removal of these proteins from CWF. Critically, one-dimensional and 2D gel electrophoresis indicated that subsequent removal of these proteins enhanced the ability to detect proteins in low abundance in CWF. Further, MS demonstrated that depletion of these abundant proteins increased the detection of other proteins in these samples. Conclusions  Results obtained indicate that this approach significantly improves separation of proteins present in low concentrations in CWF. This will facilitate the identification of biomarkers in samples collected from patients with ulcers and lead to improved patient therapies and wound care approaches.
  • References (44)
  • Citations (34)
Cite
References44
Newest
Published on May 3, 2006in Journal of Advanced Nursing 2.27
Catherine Walshe14
Estimated H-index: 14
(East Sussex County Council)
This study aimed to describe the experience of living with a venous leg ulcer from the patient's perspective, to answer the question 'What is it like to live with a venous leg ulcer?' Investigating the experience of illness is important in understanding how disease processes affect people, how they understand and cope with these processes, and how care may be given most effectively. Little research was identified which examined these areas with reference to people with leg ulceration. A qualitat...
207 Citations Source Cite
Published on Feb 1, 2006in Current Opinion in Chemical Biology 7.57
Hyoung Joo Lee15
Estimated H-index: 15
(Yonsei University),
Eun Y. Lee41
Estimated H-index: 41
(Yonsei University)
+ 1 AuthorsYoung-Ki Paik41
Estimated H-index: 41
(Yonsei University)
Because biomarkers are typically low in abundance, the crucial step of biomarker discovery is to efficiently separate clinically relevant sets of proteins that might define disease stages and/or predict disease development. It is anticipated that a multi-dimensional fractionation system (MDFS) will provide an efficient means of separating low abundance proteins from plasma proteins, resulting in the extension of the detection limit. However, when using an MDFS to analyze the plasma proteome it i...
87 Citations Source Cite
Published on Jan 1, 2006in Molecular & Cellular Proteomics 5.24
Simon Sheng4
Estimated H-index: 4
(Johns Hopkins University),
Dawn Chen5
Estimated H-index: 5
(Johns Hopkins University),
Jennifer E. Van Eyk62
Estimated H-index: 62
In biomarker discovery, the detection of proteins with low abundance in the serum proteome can be achieved by optimization of protein separation methods as well as selective depletion of the higher abundance proteins such as immunoglobins (e.g. IgG) and albumin. A relative newcomer to the proteomic separation arena is the commercial instrument PF2D from Beckman Coulter that separates proteins in the first dimension using chromatofocusing followed in line by reversed phase chromatography in the s...
93 Citations Source Cite
Published on Oct 1, 2005in Journal of Proteome Research 3.95
James Martosella5
Estimated H-index: 5
,
Nina Zolotarjova6
Estimated H-index: 6
+ 2 AuthorsBarry E. Boyes4
Estimated H-index: 4
Serum analysis represents an extreme challenge due to the dynamic range of the proteins of interest, and the high structural complexity of the constituent proteins. In serum, the quantities of proteins and peptides of interest range from those considered “high abundance”, present at 2−70% by mass of total protein, to those considered “low abundance”, present at 10-12 M or less. This range of analytical target molecules is outside the realm of available technologies for proteomic analysis. Theref...
107 Citations Source Cite
Published on Aug 1, 2005in Proteomics 3.53
Alex J. Rai14
Estimated H-index: 14
(Johns Hopkins University School of Medicine),
Craig A. Gelfand1
Estimated H-index: 1
+ 13 AuthorsHarald Tammen16
Estimated H-index: 16
There is a substantial list of pre-analytical variables that can alter the analysis of blood-derived samples. We have undertaken studies on some of these issues including choice of sample type, stability during storage, use of protease inhibitors, and clinical standardization. As there is a wide range of sample variables and a broad spectrum of analytical techniques in the HUPO PPP effort, it is not possible to define a single list of pre-analytical standards for samples or their processing. We ...
419 Citations Source Cite
Published on Aug 1, 2005in Proteomics 3.53
Nina Zolotarjova6
Estimated H-index: 6
(Agilent Technologies),
James Martosella5
Estimated H-index: 5
(Agilent Technologies)
+ 3 AuthorsWilliam C. Barrett1
Estimated H-index: 1
(Agilent Technologies)
The need to identify protein or peptide biomarkers via readily available biological samples like serum, plasma, or cerebrospinal fluid is often hindered by a few particular proteins present at relatively high concentrations. The ability to remove these proteins specifically, reproducibly, and with high selectivity is increasingly important in proteomic studies, and success in this procedure is leading to an ever-increasing list of lower abundant proteins being identified in these biological flui...
227 Citations Source Cite
Published on Aug 1, 2005in Proteomics 3.53
Lynn A. Echan6
Estimated H-index: 6
(Wistar Institute),
Hsin-Yao Tang20
Estimated H-index: 20
(Wistar Institute)
+ 2 AuthorsDavid W. Speicher64
Estimated H-index: 64
(Wistar Institute)
Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently commercialized HPLC column containing antibodies to six of the most abundant blood proteins (“Top-6 depletion”) with either older Cibacron blue/Protein A or G depletion methods or no de...
282 Citations Source Cite
Published on Aug 1, 2005in Proteomics 3.53
Ping He3
Estimated H-index: 3
(Peking Union Medical College),
Hong-Zhi He4
Estimated H-index: 4
(Peking Union Medical College)
+ 13 AuthorsKunWang5
Estimated H-index: 5
We have investigated the serum proteome of Han-nationality Chinese by using shotgun strategy. A complete proteomics analysis was performed on two reference specimens from a total of 20 healthy donors, in which each sample was made from ten-pooled male or female serum, respectively. The methodology used encompassed (1) removal of six high-abundant proteins; (2) tryptic digestion of low- and high-abundant proteins of serum; (3) separation of peptide mixture by RP-HPLC followed by ESI-MS/MS identif...
67 Citations Source Cite
Published on Aug 1, 2005in Proteomics 3.53
Sang Yun Cho19
Estimated H-index: 19
(Yonsei University),
Eun Y. Lee41
Estimated H-index: 41
(Yonsei University)
+ 12 AuthorsJin Young Kim9
Estimated H-index: 9
Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high-abundance proteins that seriously interfere with the detection of low-abundance potential biomarker proteins. To establish a high-throughput method for efficient depletion of high-abundance proteins and subsequent fractionation, prior to molecular analysis of proteins, we explored how coupled immunoaffinity columns, commercially available...
111 Citations Source Cite
Published on Jul 1, 2005in Proteomics 3.53
Monica Soldi2
Estimated H-index: 2
(University of Milano-Bicocca),
Cecilia Sarto14
Estimated H-index: 14
(University of Milano-Bicocca)
+ 4 AuthorsPaolo Mocarelli15
Estimated H-index: 15
(University of Milano-Bicocca)
Two-dimensional liquid chromatography separation (2-DL), based on chromatofocusing for first dimension and hydrophobicity for second, can be used as a complementary method to two-dimensional gel electrophoresis (2-DE). A platform now available, ProteomeLab PF 2D provided by Beck-man Coulter, (Fullerton, CA, USA), assembles these methods in automation. This system was applied to resolve large numbers of urine proteins. Reproducibility and sensitivity in protein resolution were evaluated in this s...
51 Citations Source Cite
Cited By34
Newest
Published on Feb 1, 2019in Journal of Proteomics 3.72
Nayara Rodrigues Vieira Cavassan1
Estimated H-index: 1
(Sao Paulo State University),
Caio Cavassan de Camargo3
Estimated H-index: 3
(Sao Paulo State University)
+ 5 AuthorsLucilene Delazari dos Santos16
Estimated H-index: 16
(Sao Paulo State University)
Abstract Chronic venous ulcers affect the quality of life of patients around the world. The aims of this study were to identify the proteins expressed in chronic venous ulcer exudates, to categorize them according to their roles and to correlate them with the clinical and epidemiological aspects of the disease. The study population consisted of 37 ulcers from 28 patients, and the inflammatory exudates of these thirty-seven ulcers were subjected to tryptic digestion and mass spectrometry analysis...
Source Cite
Published on Dec 2, 2018in Expert Review of Proteomics 3.49
Cristina Herrera6
Estimated H-index: 6
(University of Costa Rica),
Teresa Escalante26
Estimated H-index: 26
(University of Costa Rica)
+ 2 AuthorsJosé María Gutiérrez71
Estimated H-index: 71
(University of Costa Rica)
ABSTRACTIntroduction: Metalloproteinases play key roles in health and disease, by generating novel proteoforms with variable structure and function.Areas covered: This review focuses on the role of endogenous [a Disintegrin and Metalloproteinase (ADAMs), ADAMs with thrombospondin motifs (ADAMTS), and matrix metalloproteinases (MMPs)] and exogenous metalloproteinases in various disease conditions, and describes the application of mass spectrometry-based proteomics to detect qualitative and quanti...
1 Citations Source Cite
Published on Oct 30, 2017in Cochrane Database of Systematic Reviews 6.75
Maggie J Westby4
Estimated H-index: 4
,
Jo C Dumville36
Estimated H-index: 36
+ 4 AuthorsRichard D Riley37
Estimated H-index: 37
(Keele University)
This is a protocol for a Cochrane Review (Prognosis). The objectives are as follows: To determine whether protease activity is an independent prognostic factor for the healing of venous leg ulcers.
1 Citations Source Cite
Published on Jan 1, 2017
Q. Zeng1
Estimated H-index: 1
(State University of New York System),
L.K. Macri1
Estimated H-index: 1
(State University of New York System)
+ 10 AuthorsDario Stupar2
Estimated H-index: 2
The integration of healing, cell biology, and skin tissue engineering research has been ongoing for nearly one century. In this chapter, we provide a bird’s eye view of skin anatomy and functions, wound healing processes, the challenges and solutions to wound healing. Many techniques and biomaterials have been examined for their potential utility as skin substitutes. Notwithstanding evidence that some strategies have been more successful than others, the ideal skin substitute does not exist. Exi...
Source Cite
Published on Oct 22, 2016in Toxins 3.27
José María Gutiérrez71
Estimated H-index: 71
,
Teresa Escalante26
Estimated H-index: 26
+ 2 AuthorsJay W. Fox52
Estimated H-index: 52
Snake venom metalloproteinases (SVMPs) affect the extracellular matrix (ECM) in multiple and complex ways. Previously, the combination of various methodological platforms, including electron microscopy, histochemistry, immunohistochemistry, and Western blot, has allowed a partial understanding of such complex pathology. In recent years, the proteomics analysis of exudates collected in the vicinity of tissues affected by SVMPs has provided novel and exciting information on SVMP-induced ECM altera...
13 Citations Source Cite
Published on Oct 1, 2016in International Wound Journal 2.38
Sarah Ramsay3
Estimated H-index: 3
(Smith & Nephew),
Linda Cowan6
Estimated H-index: 6
+ 2 AuthorsGregory S. Schultz61
Estimated H-index: 61
(University of Florida)
Chronic wounds, including diabetic foot ulcers, pressure ulcers and venous leg ulcers, impact the lives of millions of people worldwide. These types of wounds represent a significant physical, social and financial burden to both patients and health care systems. Wound care has made great progress in recent years as a result of the critical research performed in academic, clinical and industrial settings. However, there has been relatively little translation of basic research discoveries into nov...
6 Citations Source Cite
Published on Sep 1, 2016in Journal of Proteomics 3.72
Tuo Zang2
Estimated H-index: 2
(Queensland University of Technology),
Daniel Broszczak4
Estimated H-index: 4
(Queensland University of Technology)
+ 3 AuthorsTony J. Parker6
Estimated H-index: 6
(Queensland University of Technology)
Abstract Burn injury is highly traumatic for paediatric patients, with the severity of the burn often dictating the extent of scar formation. The diagnosis of burn wound severity is largely determined by the attending clinician's experience. Thus, a greater understanding of the biochemistry at burn wound site environment and the biology of burns of different severities at an earlier stage may reduce the reliance on subjective diagnoses. In this study, blister fluid was collected from superficial...
3 Citations Source Cite
Published on Jan 1, 2016
Anthony R. Sheets5
Estimated H-index: 5
,
Catalina K. Hwang1
Estimated H-index: 1
,
Ira M. Herman43
Estimated H-index: 43
Nonhealing cutaneous ulcers that occur in diabetes, vascular insufficiency, and other chronic conditions, are a global health concern and continue to increase in annual incidence. Despite the volume of preclinical and clinically significant findings regarding either the molecular and cellular mechanisms driving wound chronicity or treatment strategies that aim to couple wound diagnostics with treatment strategies, existing therapeutics have not matched the enormous, unmet global need for advance...
1 Citations Source Cite
Published on Aug 1, 2015in Rechtsmedizin 0.64
M. Fattouh , Claas T. Buschmann11
Estimated H-index: 11
(Charité)
+ 3 AuthorsMichael Tsokos31
Estimated H-index: 31
(Charité)
Hintergrund Die Expression leukozytarer und endothelialer Adhasionsmolekule aus der Gruppe der Selektin-, Integrin- und der Immunglobulinsuperfamilie in primar heilenden Hautwunden ist tierexperimentell sowie an humanem Operations- und Autopsiematerial untersucht worden. Fur die Dekubituswunde existieren bezuglich der Gewebeexpression leukozytarer und endothelialer Adhasionsmolekule bisher allerdings keine gesicherten Erkenntnisse.
Source Cite
Published on Aug 1, 2015in Burns 2.13
Catherine Tanzer2
Estimated H-index: 2
,
Dayle L. Sampson3
Estimated H-index: 3
(Queensland University of Technology)
+ 5 AuthorsTony J. Parker6
Estimated H-index: 6
(Queensland University of Technology)
a b s t r a c t The early and accurate assessment of burns is essential to inform patient treatment regimens; however, this first critical step in clinical practice remains a challenge for specialist burns clinicians worldwide. In this regard, protein biomarkers are a potential adjunct diagnostic tool to assist experienced clinical judgement. Free circulating haemoglobin has previously shown some promise as an indicator of burn depth in a murine animal model. Using blister fluid collected from p...
3 Citations Source Cite