Original paper
Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters
Abstract
The cloning of long DNA segments, especially those containing large gene clusters, is of particular importance to synthetic and chemical biology efforts for engineering organisms. While cloning has been a defining tool in molecular biology, the cloning of long genome segments has been challenging. Here we describe a technique that allows the targeted cloning of near-arbitrary, long bacterial genomic sequences of up to 100 kb to be accomplished...
Paper Details
Title
Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters
Published Date
Sep 1, 2015
Journal
Volume
6
Issue
1
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Notes
History