Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
Abstract
Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity. In loop-mediated isothermal amplification (LAMP)...
Paper Details
Title
Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
Published Date
Feb 1, 2015
Journal
Volume
58
Issue
2
Pages
59 - 68
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