A generic high-throughput assay to detect aquaporin functional mutants: Potential application to discovery of aquaporin inhibitors

The discovery of stable, yet functional, protein mutants is a limiting factor in the development of biotechnological applications, structural studies or in drug discovery. Rapid detection of functional mutants is especially challenging for water channel aquaporins, as they do not have a directly measurable enzymatic or binding activity. Current methods available are time consuming and only applicable to specific aquaporins.Herein we describe an...