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A novel role of Drosophila cytochrome P450-4e3 in permethrin insecticide tolerance

Published on Dec 1, 2015in Insect Biochemistry and Molecular Biology3.62
· DOI :10.1016/j.ibmb.2015.06.002
Selim Terhzaz19
Estimated H-index: 19
(Glas.: University of Glasgow),
Pablo Cabrero19
Estimated H-index: 19
(Glas.: University of Glasgow)
+ 3 AuthorsShireen A. Davies36
Estimated H-index: 36
(Glas.: University of Glasgow)
Cite
Abstract
The exposure of insects to xenobiotics, such as insecticides, triggers a complex defence response necessary for survival. This response includes the induction of genes that encode key Cytochrome P450 monooxygenase detoxification enzymes. Drosophila melanogaster Malpighian (renal) tubules are critical organs in the detoxification and elimination of these foreign compounds, so the tubule response induced by dietary exposure to the insecticide permethrin was examined. We found that expression of the gene encoding Cytochrome P450-4e3 (Cyp4e3) is significantly up-regulated by Drosophila fed on permethrin and that manipulation of Cyp4e3 levels, specifically in the principal cells of the Malpighian tubules, impacts significantly on the survival of permethrin-fed flies. Both dietary exposure to permethrin and Cyp4e3 knockdown cause a significant elevation of oxidative stress-associated markers in the tubules, including H2O2 and lipid peroxidation byproduct, HNE (4-hydroxynonenal). Thus, Cyp4e3 may play an important role in regulating H2O2 levels in the endoplasmic reticulum (ER) where it resides, and its absence triggers a JAK/STAT and NF-κB-mediated stress response, similar to that observed in cells under ER stress. This work increases our understanding of the molecular mechanisms of insecticide detoxification and provides further evidence of the oxidative stress responses induced by permethrin metabolism.
  • References (40)
  • Citations (21)
Cite
References40
Newest
Published on Apr 1, 2013in The FASEB Journal5.39
Svetlana N. Radyuk17
Estimated H-index: 17
(SMU: Southern Methodist University),
Vladimir I. Klichko14
Estimated H-index: 14
(SMU: Southern Methodist University)
+ 1 AuthorsWilliam C. Orr32
Estimated H-index: 32
(SMU: Southern Methodist University)
Peroxiredoxin 4 (Prx4) has been implicated in a wide variety of biological processes, including development, progression of cancer, inflammation, and antioxidant function. The purpose of this study was to provide further insight into its multiple roles at the whole-animal level, using Drosophila. Reduced expression of dPrx4 (up to 90%) resulted in greater sensitivity to oxidative stress, an elevated H2O2 flux, and increases in lipid peroxidation, but no effect on longevity. Overexpression at low...
Published on Jun 29, 2012in PLOS ONE2.78
Predrag Kalajdzic5
Estimated H-index: 5
(University of Belgrade),
Stefan Oehler5
Estimated H-index: 5
+ 4 AuthorsCharalambos Savakis29
Estimated H-index: 29
(UoC: University of Crete)
Insecticide resistance is a worldwide problem with major impact on agriculture and human health. Understanding the underlying molecular mechanisms is crucial for the management of the phenomenon; however, this information often comes late with respect to the implementation of efficient counter-measures, particularly in the case of metabolism-based resistance mechanisms. We employed a genome-wide insertional mutagenesis screen to Drosophila melanogaster, using a Minos-based construct, and retriev...
Published on Apr 4, 2012in PLOS ONE2.78
Venkateswara R. Chintapalli9
Estimated H-index: 9
(Glas.: University of Glasgow),
Selim Terhzaz19
Estimated H-index: 19
(Glas.: University of Glasgow)
+ 5 AuthorsJulian A. T. Dow48
Estimated H-index: 48
(Glas.: University of Glasgow)
In humans and other animals, the internal organs are positioned asymmetrically in the body cavity, and disruption of this body plan can be fatal in humans. The mechanisms by which internal asymmetry are established are presently the subject of intense study; however, the functional significance of internal asymmetry (outside the brain) is largely unexplored. Is internal asymmetry functionally significant, or merely an expedient way of packing organs into a cavity?
Published on Jan 11, 2012in PLOS ONE2.78
Selim Terhzaz19
Estimated H-index: 19
(Glas.: University of Glasgow),
Pablo Cabrero19
Estimated H-index: 19
(Glas.: University of Glasgow)
+ 5 AuthorsShireen A. Davies36
Estimated H-index: 36
(Glas.: University of Glasgow)
The capa peptide receptor, capaR (CG14575), is a G-protein coupled receptor (GPCR) for the D. melanogaster capa neuropeptides, Drm-capa-1 and -2 (capa-1 and -2). To date, the capa peptide family constitutes the only known nitridergic peptides in insects, so the mechanisms and physiological function of ligand-receptor signalling of this peptide family are of interest. Capa peptide induces calcium signaling via capaR with EC50 values for capa-1 = 3.06 nM and capa-2 = 4.32 nM. capaR undergoes rapid...
Published on Jan 1, 2012
S M D Akbar4
Estimated H-index: 4
,
C SharmaHari30
Estimated H-index: 30
+ 1 AuthorsK. Sreeramulu14
Estimated H-index: 14
The cotton bollworm, Helicoverpa armigera is a polyphagous pest of several crops in Asia, Africa, and the Mediterranean Europe. Pyrethroid insecticides are used to control noxious insects in agriculture, forestry, households and horticulture. Therefore, the effect of permethrin and fenvalarate was evaluated on the oxidative stress in H. armigera. Lipid peroxidation, H2O2 content, and lactate dehydrogenase (LDH) activity increased and glutathione reductase (GR) activity decreased in a time- and d...
Published on Dec 1, 2011in Cell Metabolism22.41
Simone C. Albrecht2
Estimated H-index: 2
(DKFZ: German Cancer Research Center),
Ana G. Barata3
Estimated H-index: 3
(DKFZ: German Cancer Research Center)
+ 2 AuthorsTobias P. Dick34
Estimated H-index: 34
(DKFZ: German Cancer Research Center)
Summary The glutathione redox couple (GSH/GSSG) and hydrogen peroxide (H 2 O 2 ) are central to redox homeostasis and redox signaling, yet their distribution within an organism is difficult to measure. Using genetically encoded redox probes in Drosophila , we establish quantitative in vivo mapping of the glutathione redox potential ( E GSH ) and H 2 O 2 in defined subcellular compartments (cytosol and mitochondria) across the whole animal during development and aging. A chemical strategy to trap...
Published on Sep 1, 2011in Cardiovascular Toxicology2.63
Dhivya Vadhana1
Estimated H-index: 1
,
Manuel Carloni10
Estimated H-index: 10
+ 2 AuthorsRosita Gabbianelli19
Estimated H-index: 19
Due to increased global use, acute and chronic exposures to pyrethroid insecticides in humans are of clinical concern. Pyrethroids have a primary mode of action that involves interference with the sodium and calcium channels in excitable cells, which may include cardiac myocytes. Here, we investigated the possible cardiac toxicity of permethrin metabolites (METP), 3-phenoxy-benzyl alcohol (3PBA), 3-phenoxy-benzaldehyde (3PBALD), and 3-phenoxybenzoic acid (3PBACID). Plasma membrane fluidity, pola...
Published on May 1, 2010in Cellular Signalling3.39
Selim Terhzaz19
Estimated H-index: 19
(Glas.: University of Glasgow),
Andrew J. Finlayson1
Estimated H-index: 1
(Glas.: University of Glasgow)
+ 5 AuthorsShireen A. Davies36
Estimated H-index: 36
(Glas.: University of Glasgow)
Abstract Organismal stress responses to oxidative stress are relevant to ageing and disease and involve key cell-/tissue-specific signal transduction mechanisms. Using Drosophila , an established in vivo model for stress studies, we show that cell-specific inositol phosphate signalling specifically via inositol 1,4,5 trisphosphate 3-kinase (InsP 3 3-K, IP 3 K), negatively regulates organismal responses to oxidative stress. We demonstrate that the Drosophila Malpighian tubule (equivalent to verte...
Published on Mar 1, 2010in Environmental Toxicology and Chemistry3.42
Fen Hu3
Estimated H-index: 3
(WMU: Wenzhou Medical College),
Ling Li11
Estimated H-index: 11
(Zhejiang University of Technology)
+ 3 AuthorsMeirong Zhao22
Estimated H-index: 22
(Zhejiang University of Technology)
Synthetic pyrethroid (SP) insecticides are chiral compounds with multiple asymmetric positions. Several recent studies have focused on the effect of enantioselectivity of SPs in acute aquatic toxicity, endocrine-disrupting activities, and immunotoxicity. However, the relevant molecular mechanisms are still unknown. The potential relationship between ecotoxicological effects and oxidative stress could contribute to SP-induced enantioselective cytotoxicity, but this requires further investigation....
Published on Nov 13, 2009in Journal of Biological Chemistry
Marcus Gutscher3
Estimated H-index: 3
(DKFZ: German Cancer Research Center),
Mirko C. Sobotta7
Estimated H-index: 7
(DKFZ: German Cancer Research Center)
+ 4 AuthorsTobias P. Dick34
Estimated H-index: 34
H2O2 acts as a signaling molecule by oxidizing critical thiol groups on redox-regulated target proteins. To explain the efficiency and selectivity of H2O2-based signaling, it has been proposed that oxidation of target proteins may be facilitated by H2O2-scavenging peroxidases. Recently, a peroxidase-based protein oxidation relay has been identified in yeast, namely the oxidation of the transcription factor Yap1 by the peroxidase Orp1. It has remained unclear whether the protein oxidase function ...
Cited By21
Newest
Published on 2019in Pesticide Biochemistry and Physiology2.87
Kai Lu1
Estimated H-index: 1
(Fujian Agriculture and Forestry University),
Yibei Cheng1
Estimated H-index: 1
(Fujian Agriculture and Forestry University)
+ 7 AuthorsTingting Liu (Fujian Agriculture and Forestry University)
Abstract Environmental xenobiotics can influence the tolerance of insects to chemical insecticides. Heavy metals are widespread distributed, can be easily bio-accumulated in plants and subsequently within phytophagous insects via the food chains. However, less attention has been paid to the effect of heavy metal exposure on their insecticide tolerance. In this study, pre-exposure of copper (Cu, 25–100 mg kg−1) significantly enhanced the subsequent tolerance of Spodoptera litura to β-cypermethrin...
Published on Jun 1, 2019in Insect Biochemistry and Molecular Biology3.62
Leah T. Sigle (PSU: Pennsylvania State University), Elizabeth A. McGraw31
Estimated H-index: 31
(PSU: Pennsylvania State University)
Abstract Mosquito transmitted viruses cause significant morbidity and mortality in human populations. Despite the use of insecticides and other measures of vector control, arboviral diseases are on the rise. One potential solution for limiting disease transmission to humans is to render mosquitoes refractory to viral infection through genetic modification. Substantial research effort in Drosophila , Aedes and Anopheles has helped to define the major innate immune pathways, including Toll, IMD, J...
Osvaldo Rivera1
Estimated H-index: 1
(ULM: University of Louisiana at Monroe),
Lara McHan (ULM: University of Louisiana at Monroe)+ 3 AuthorsMatthew E. Talbert7
Estimated H-index: 7
(ULM: University of Louisiana at Monroe)
Obesity predisposes humans to a range of life-threatening comorbidities, including type 2 diabetes and cardiovascular disease. Obesity also aggravates neural pathologies, such as Alzheimer’s disease, but this class of comorbidity is less understood. When Drosophila melanogaster (flies) are exposed to high-fat diet (HFD) by supplementing a standard medium with coconut oil, they adopt an obese phenotype of decreased lifespan, increased triglyceride storage, and hindered climbing ability. The latte...
Published on Apr 1, 2019in Pest Management Science3.25
Chengyu Chen4
Estimated H-index: 4
(CAU: China Agricultural University),
Tisheng Shan (CAU: China Agricultural University)+ 2 AuthorsXiwu Gao26
Estimated H-index: 26
(CAU: China Agricultural University)
Published on Mar 12, 2019in PLOS ONE2.78
Tsering Stobdan15
Estimated H-index: 15
,
Debashis Sahoo25
Estimated H-index: 25
+ 4 AuthorsGabriel G. Haddad54
Estimated H-index: 54
Published on Jan 29, 2019in PLOS ONE2.78
Corey L. Campbell19
Estimated H-index: 19
,
Karla Saavedra-Rodriguez13
Estimated H-index: 13
+ 3 AuthorsWilliam C. Black51
Estimated H-index: 51
Published on Dec 1, 2018in BMC Genomics3.50
Er-Hu Chen7
Estimated H-index: 7
(SWU: Southwest University),
Qiu-Li Hou4
Estimated H-index: 4
(SWU: Southwest University)
+ 7 AuthorsJinjun Wang27
Estimated H-index: 27
(SWU: Southwest University)
The oriental fruit fly, Bactrocera dorsalis (Hendel) has been considered to be one of the most important agricultural pest around the world. As a holometabolous insect, larvae must go through a metamorphosis process with dramatic morphological and structural changes to complete their development. To better understand the molecular mechanisms of these changes, RNA-seq of B. dorsalis from wandering stage (WS), late wandering stage (LWS) and white puparium stage (WPS) were performed. In total, 11,7...
Published on Dec 1, 2018in Experimental Neurology4.56
Yuuka Muraoka2
Estimated H-index: 2
(Kyoto Institute of Technology),
Aya Nakamura1
Estimated H-index: 1
(Kyoto Institute of Technology)
+ 10 AuthorsTakahiko Tokuda34
Estimated H-index: 34
(Kyoto Prefectural University of Medicine)
Abstract Neuron-specific knockdown of the dFIG4 gene, a Drosophila homologue of human FIG4 and one of the causative genes for Charcot-Marie-Tooth disease (CMT), reduces the locomotive abilities of adult flies, as well as causing defects at neuromuscular junctions, such as reduced synaptic branch length in presynaptic terminals of the motor neurons in third instar larvae. Eye imaginal disc-specific knockdown of dFIG4 induces abnormal morphology of the adult compound eye, the rough eye phenotype. ...