[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

Volume: 12, Pages: 195 - 206
Published: Jan 1, 1968
Abstract
The GC content of highly purified DNA samples can be estimated from their thermal denaturation temperatures, employing small amounts of DNA (10–40 μg). The method is simple, convenient and utilizes readily available laboratory equipment; the thermal denaturation profile also gives a good picture of the quality of the DNA preparation. Denatured DNA present in native preparations, or single stranded DNA obtained by denaturing native DNA, or...
Paper Details
Title
[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA
Published Date
Jan 1, 1968
Volume
12
Pages
195 - 206
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