Carbene‐Induced Rescue of Catalytic Activity in Deactivated Nitrite Reductase Mutant

The role of His145 in the T1 copper center of nitrite reductase (NiR) is pivotal for the activity of the enzyme. Mutation to a glycine at this position enables the reconstitution of the T1 center by the addition of imidazole as exogenous ligands, however the catalytic activity is only marginally rescued. Here, we demonstrate that the uptake of 1,3‐dimethylimidazolylidene as N‐heterocyclic carbene (NHC) by the H145G NiR mutant instead of...