Chronic glucokinase activator treatment activates liver Carbohydrate response element binding protein and improves hepatocyte ATP homeostasis during substrate challenge

Aim To test the hypothesis that glucokinase activators (GKAs) induce hepatic adaptations that alter intra‐hepatocyte metabolite homeostasis. Methods C57BL/6 mice on a standard rodent diet were treated with a GKA (AZD1656) acutely or chronically. Hepatocytes were isolated from the mice after 4 or 8 weeks of treatment for analysis of cellular metabolites and gene expression in response to substrate challenge. Results Acute exposure of mice to...