Inhibitive effect of prolonged hyperoxic exposure on the protection of keratinocyte growth factor on alveolar epithelial type II cells of rat fetus

Objective To explore the survival/proliferation, apoptotic and death effects of keratinocyte growth factor (KGF) in alveolar epithelial type Ⅱ cells (ATⅡCs) exposed to hyperoxia. Methods Primary culture of ATⅡCs from the Sprague-Dawley rat fetuses was studied under room air condition (210 mL/L O2) and hyperoxic condition (950 mL/L O2) for 0.5-12.0 h. Various concentrations of KGF (15 μg/L, 25 μg/L, 50 μg/L, 75 μg/L, 100 μg/L) were added...