A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Abstract
Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE variant-YE1-BE3-FNLS-that retains high on-target...
Paper Details
Title
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Published Date
May 18, 2020
Journal
Volume
17
Issue
6
Pages
600 - 604
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