Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
Abstract
CRISPR RNA-guided endonucleases (RGEs) cut or direct activities to specific genomic loci, yet each has off-target activities that are often unpredictable. We developed a pair of simple in vitro assays to systematically measure the DNA-binding specificity (Spec-seq), catalytic activity specificity (SEAM-seq) and cleavage efficiency of RGEs. By separately quantifying binding and cleavage specificity, Spec/SEAM-seq provides detailed mechanistic...
Paper Details
Title
Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
Published Date
Apr 21, 2020
Journal
Volume
48
Issue
9
Pages
5037 - 5053
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