A high-resolution measurement of nucleotide sugars by using ion-pair reverse chromatography and tandem columns

Volume: 412, Issue: 15, Pages: 3683 - 3693
Published: Apr 16, 2020
Abstract
N-Linked glycosylation is a cellular process transferring sugars from glycosyl donors to proteins or lipids. Biopharmaceutical products widely produced by culturing mammalian cells such as Chinese hamster ovary (CHO) cells are typically glycosylated during biosynthesis. For some biologics, the N-linked glycan is a critical quality attribute of the drugs. Nucleotide sugars are the glycan donors and impact the intracellular glycosylation process....
Paper Details
Title
A high-resolution measurement of nucleotide sugars by using ion-pair reverse chromatography and tandem columns
Published Date
Apr 16, 2020
Volume
412
Issue
15
Pages
3683 - 3693
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