PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells
Abstract
We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Forster resonance energy transfer (FRET) to detect changes in biosensor conformation that accompany the targeted cell signaling event. One of the most robust ways of quantifying FRET...
Paper Details
Title
PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells
Published Date
Apr 1, 2020
Journal
Volume
118
Issue
8
Pages
1820 - 1829
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