New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

Here, we experimentally expand understanding of the reactions and enzymes involved in A. thiooxidans ATCC 19377 S0 and S2O32- metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans S2O32- metabolism model involves the conversion of S2O32- to SO42-, S0 and S4O62-, mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase...