Releasing the microbes from old bones: the effect of different DNA extraction protocols on microbial community profiling

DNA-based characterisation of microbial communities can enable those interested in bone diagenesis to address questions relating to the complexity and diversity of said microbial communities. We explored whether biases could be introduced due to differences in the DNA extraction methods used. We investigated the effect of four alternative approaches on the extraction of DNA from bone, in order to examine the resulting effect on the bacterial and...