Updated emm-typing protocol for Streptococcus pyogenes

Volume: 26, Issue: 7, Pages: 946.e5 - 946.e8
Published: Jul 1, 2020
Abstract
ObjectivesPCR-based typing of the emm gene Streptococcus pyogenes often results in the amplification of multiple bands. This has resulted in the misclassification of strains into types based on non-emm gene sequences. We aimed to improve the specificity of the emm typing PCR reaction using a primer called CDC3, the sequence for which has been previously used to identify emm genes in silico.MethodsThe proposed primer CDC3 was validated in silico...
Paper Details
Title
Updated emm-typing protocol for Streptococcus pyogenes
Published Date
Jul 1, 2020
Volume
26
Issue
7
Pages
946.e5 - 946.e8
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