Gold nanoparticles enumeration with dark-field optical microscope for the sensitive glycoprotein sandwich assay

Protein glycosylation is an important post-translational modification and glycoproteins are associated with many crucial metabolic progresses of life. In order to detect glycoproteins sensitively, we propose a gold nanoparticles (GNPs) enumeration method based on boronate affinity sandwich system, which is constructed between the boronic acid polymer functionalized magnetic nanoparticles (Fe3O4@[email protected] NPs) and 4-mercaptophenylboronic...