A cost-effective and rapid aptasensor with chemiluminescence detection for the early diagnosis of prostate cancer

A rapid aptasensor capable of early diagnosing prostate cancer was developed using a dual aptamer, which is composed of a prostate specific antigen (PSA) aptamer and hemin aptamer connected by a linker composed of 5 adenines. The dual aptamers immobilized on the surface of paramagnetic beads were able to competitively bind with PSA or hemin in human serum for 20 min at room temperature. After the incubation, the dual aptamers were separated from...