Test validation, method comparison and reference range for the measurement of β-hydroxybutyrate in peripheral blood samples

The measurement of β-hydroxybutyrate (βOHB) concentrations is a corner stone of the diagnosis of diabetic ketoacidosis and other ketonic states. The aim of this study was to perform a validation of a peripheral blood βOHB assay (Randox) on a Roche cobas c502 analyser and to establish a βOHB reference range for the validated assay.Precision, linearity and limit of detection and blank (LoD, LoB) were determined according to Clinical and Laboratory...