The Dissociation Mechanism of the Processive Cellulase TrCel7A

Cellulase enzymes catalyze the hydrolysis of recalcitrant cellulose into soluble sugars, and are thus a key biocatalyst in the growinglignocellulosic bioeconomy. Cel7A from Trichoderma reesei (TrCel7A) is a widely used model enzyme typical for this class of cellobiohydrolases (CBH). It processively releases many sugar molecules without dissociating from the substrate. However, dissociation from the cellulose substrate to avoid barriers along the...