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The microbiota programs DNA methylation to control intestinal homeostasis and inflammation

Published on Feb 3, 2020in Nature microbiology14.633
· DOI :10.1038/S41564-019-0659-3
Ihab Ansari1
Estimated H-index: 1
(HUJI: Hebrew University of Jerusalem),
Günter Raddatz10
Estimated H-index: 10
(DKFZ: German Cancer Research Center)
+ 9 AuthorsEran Elinav42
Estimated H-index: 42
(Weizmann Institute of Science)
Sources
Abstract
Although much research has been done on the diversity of the gut microbiome, little is known about how it influences intestinal homeostasis under normal and pathogenic conditions. Epigenetic mechanisms have recently been suggested to operate at the interface between the microbiota and the intestinal epithelium. We performed whole-genome bisulfite sequencing on conventionally raised and germ-free mice, and discovered that exposure to commensal microbiota induced localized DNA methylation changes at regulatory elements, which are TET2/3-dependent. This culminated in the activation of a set of ‘early sentinel’ response genes to maintain intestinal homeostasis. Furthermore, we demonstrated that exposure to the microbiota in dextran sodium sulfate-induced acute inflammation results in profound DNA methylation and chromatin accessibility changes at regulatory elements, leading to alterations in gene expression programs enriched in colitis- and colon-cancer-associated functions. Finally, by employing genetic interventions, we show that microbiota-induced epigenetic programming is necessary for proper intestinal homeostasis in vivo. A comparison of DNA methylation patterns between conventionally raised and germ-free mice shows that the presence of a commensal microbiota induces methylation changes at regulatory elements in a subset of genes that are important for intestinal homeostasis.
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