Cellular uptake of N-acetyl-d-galactosamine-, N-acetyl-d-glucosamine- and d-mannose-containing fluorescent glycoconjugates investigated by liver intravital microscopy

• Three branched N -acetyl- d -galactosamine-, N -acetyl- d -glucosamine- and d -mannose derivatives were synthesized. • The obtained compounds were conjugated to fluorescent dye sulfo -Cy5 alkyne. • By liver intravital microscopy, cellular uptake of the fluorescent glycoconjugates was tracked in real time. • GalNAc conjugate was taken up by hepatocytes, while GlcNAc and Man derivatives entered liver sinusoidal endothelial...