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Synthesis of bioactive material by sol–gel process utilizing polymorphic calcium carbonate precipitate and their direct and indirect in-vitro cytotoxicity analysis

Published on May 1, 2020in Environmental Technology and Innovation2.8
· DOI :10.1016/J.ETI.2020.100647
Manish Kumar23
Estimated H-index: 23
(JNU: Jawaharlal Nehru University),
Manish Kumar6
Estimated H-index: 6
(JNU: Jawaharlal Nehru University)
+ 0 AuthorsIndu Shekhar Thakur27
Estimated H-index: 27
(JNU: Jawaharlal Nehru University)
Abstract
Abstract To mitigate the rising level of CO2, biological method of CO2 sequestration is one of the effective methods. Chemolithotrophic microbes are able to fix atmospheric CO2 and precipitated polymorphic minerals like calcite, vaterite and aragonite. Based on this, bioactive glass was synthesized by sol–gel process using polymorphic calcium carbonate mineral precipitated by chemolithotrophic Serratia sp. ISTD04. Characterization of bioactive material and its bioactivity was evaluated by SEM, EDX, FT-IR, XRD, ICP-MS. SEM analysis revealed biomaterial showing more bioactivity due to deposition of smaller particle like appearance throughout the surface. FT-IR analysis of sintered and immersed bioactive material indicated presence of O-Ca-O, O-Si-O and Si-O-Si functional group. The XRD analysis indicated important features similar to melt-derived Na2O-containg glass ceramics like formation of crystalline phase Na2Ca2Si3O9. Further in-vitro study was performed in simulated body fluid (SBF) and on osteosarcoma cell line, confirmed that material and their supernatant did not reflect any cytotoxicity.
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  • Citations (3)
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References46
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#1Manish Kumar (JNU: Jawaharlal Nehru University)H-Index: 6
#2Indu Shekhar Thakur (JNU: Jawaharlal Nehru University)H-Index: 7
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#2Smita Sundaram (JNU: Jawaharlal Nehru University)H-Index: 4
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Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO3. One of the isolate showed resistance to NaHCO3 (100 mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with pr...
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ABSTRACT The Serratia sp. strain ISTD04 has been identified as a carbon dioxide (CO2)-sequestering bacterium isolated from marble mining rocks in the Umra area, Rajasthan, India. This strain grows chemolithotrophically on media that contain sodium bicarbonate (NaHCO3) as the sole carbon source. Here, we report the genome sequence of 5.07 Mb Serratia sp. ISTD04.
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