Protein retention in dextran-grafted cation exchange chromatography: The influence of pHs, counterions and polymer structure

Polymer-grafted ion exchange adsorbents were of great interest for the development of high-performance protein chromatography in biopharmaceutical and related fields. In this work, protein retention was systematically investigated in ion exchange chromatography packed respectively with dextran-grafted cation exchange adsorbents containing sulphopropyl (SP) ligand, SP Sepharose XL and Capto S, and non-grafted cation exchange adsorbent, SP...