Cell Cycle Analysis and Relevance for Single‐Cell Gating in Mass Cytometry
Abstract
Cell cycle analysis by mass cytometry (MC) is hampered by the poor resolution of the Iridium-labeled DNA intercalator compared to DNA-specific fluorescent dyes. We report here a minimum cell cycle panel for MC consisting of Ir-intercalator (DNA content), IdU (S phase), anti-pS28HistoneH3 (mitosis), anti-CDT1 (G1 phase) and anti-Geminin (non-G1 phases). Cell cycle distributions obtained by MC were not significantly different from fluorescence...
Paper Details
Title
Cell Cycle Analysis and Relevance for Single‐Cell Gating in Mass Cytometry
Published Date
Jan 14, 2020
Journal
Volume
97
Issue
8
Pages
832 - 844
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