PAD4 Promotes NLRP3 Inflammasome Assembly Leading to NETosis

Neutrophil extracellular trap formation (NETosis) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome assembly are associated with a similar spectrum of disorders. We hypothesized that NLRP3 inflammasome may participate in NETosis. Using confocal microscopy, we observed the formation of ASC speck in stimulated neutrophils in the absence of infection, as well as the association of ASC speck with the released NETs. Peptidylarginine...