Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance
Abstract
Background Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase from Piromyces (PirXI) is used but the in vivo activity is rather low and very high levels of the enzyme are needed for xylose...
Paper Details
Title
Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance
Published Date
Jan 11, 2020
Journal
Volume
13
Issue
1
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