An improved Xer-cise technology for the generation of multiple unmarked mutants in Mycobacteria
Abstract
Xer-cise is a technique using antibiotic resistance cassettes flanked by dif sites allowing spontaneous and accurate excision from bacterial chromosomes with a high frequency through the action of the cellular recombinase XerCD. Here, we report a significant improvement of Xer-cise in Mycobacteria. Zeocin resistance cassettes flanked by variants of the natural Mycobacterium tuberculosis dif site were constructed and shown to be effective tools...
Paper Details
Title
An improved Xer-cise technology for the generation of multiple unmarked mutants in Mycobacteria
Published Date
Feb 1, 2020
Journal
Volume
68
Issue
2
Pages
106 - 110
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