Localization of sperm intracellular Ca2+ keeps fertilizability in the newt vas deferens

Sperm intracellular Ca 2+ is crucial for the induction of sperm-egg interaction, but little is known about the significance of Ca 2+ maintenance prior to induction. In sperm of the newt Cynops pyrrhogaster , intracellular Ca 2+ is localized to the midpiece during storage in the vas deferens, while extracellular Ca 2+ is influxed in modified Steinberg’s salt solution to promote a spontaneous acrosome reaction related to the decline of sperm...