Cell‐Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His‐Tagged Proteins
Abstract
In current cell‐free biotechnology, mimicking physicochemical aspects of cellular life while providing efficient protein synthesis, separation, and purification is a crucial step towards truly applicable artificial, yet cell‐like, biosynthesis platforms. To achieve simultaneous synthesis and immobilization of proteins in a tailor‐made environment, we link DNA encoding for histidine‐tagged proteins to microfluidically prepared microgels via...
Paper Details
Title
Cell‐Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His‐Tagged Proteins
Published Date
Dec 23, 2019
Journal
Volume
2
Issue
3
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