Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

Volume: 63, Issue: 7, Pages: 1053 - 1062
Published: Dec 20, 2019
Abstract
CRISPR/Cas-mediated genome editing has greatly facilitated the study of gene function in Streptomyces. However, it could not be efficiently employed in streptomycetes with low homologous recombination (HR) ability. Here, a deaminase-assisted base editor dCas9-CDA-ULstr was developed in Streptomyces, which comprises the nuclease-deficient Cas9 (dCas9), the cytidine deaminase from Petromyzon marinus (PmCDA1), the uracil DNA glycosylase inhibitor...
Paper Details
Title
Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces
Published Date
Dec 20, 2019
Volume
63
Issue
7
Pages
1053 - 1062
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