PAQMAN: Protein-nucleic acid affinity quantification by MAss spectrometry in nuclear extracts
Abstract
In recent years, various mass spectrometry-based approaches have been developed to determine global protein-DNA binding specificities using DNA affinity purifications from crude nuclear extracts. However, these assays are semi-quantitative and do not provide information about interaction affinities. We recently developed a technology that we call Protein-nucleic acid Affinity Quantification by MAss spectrometry in Nuclear extracts or PAQMAN,...
Paper Details
Title
PAQMAN: Protein-nucleic acid affinity quantification by MAss spectrometry in nuclear extracts
Published Date
Dec 1, 2020
Journal
Volume
184
Pages
70 - 77
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