RNA-Seq analysis of Orobanche resistance in Nicotiana tabacum: development of molecular markers for breeding recessive tolerance from ‘Wika’ tobacco variety

Orobanche spp. (broomrape) is an obligate root parasite that can attack a wide spectrum of plants, including tobacco. It has been responsible for economic losses in Europe since 2002 and its incidence in many tobacco-growing countries is increasing. Preventive and curative methods exist, including the use of agrochemicals, however efficacy is limited and pest dissemination remains important due to a high rate of multiplication of the parasite...