Out-of-season artificial reproduction techniques of cultured female tongue sole (Cynoglossus semilaevis): Broodstock management, administration methods of hormone therapy and artificial fertilization

The present study aimed at the development of an effective and efficient artificial reproduction technique for cultured female tongue sole (Cynoglossus semilaevis) in out-of-season. After about four-month enriching cultivation of broodstocks, a series of experiments were conducted to develop a protocol for the hormonal induction of ovulation and artificial fertilization. The results showed that single injection of combination of luteinizing...